Detailed information of TA system TA135997

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazF-pemI/MazF(toxin)
• Location: 621386..622022
• Replicon: chromosome
• Accession: NZ_CP042593
• Organism: Bacillus dafuensis strain FJAT-25496

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: FSZ17_RS03220
• Protein ID: WP_057775733.1
• Coordinates: 621672..622022 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: A0A5B8Z0R5
• Locus tag: FSZ17_RS03215
• Protein ID: WP_057775732.1
• Coordinates: 621386..621667 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FSZ17_RS03195	617099..617701	-	603	WP_057775728.1	rhomboid family intramembrane serine protease	-
FSZ17_RS03200	618179..618529	+	351	WP_057775729.1	holo-ACP synthase	-
FSZ17_RS03205	618872..619879	+	1008	WP_057775730.1	outer membrane lipoprotein carrier protein LolA	-
FSZ17_RS03210	620012..621181	+	1170	WP_057775731.1	alanine racemase	-
FSZ17_RS03215	621386..621667	+	282	WP_057775732.1	CopG family ribbon-helix-helix protein	Antitoxin
FSZ17_RS03220	621672..622022	+	351	WP_057775733.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
FSZ17_RS03225	622390..624585	+	2196	WP_185150661.1	RNA-binding transcriptional accessory protein	-
FSZ17_RS03230	624719..624832	-	114	WP_146846361.1	cortex morphogenetic protein CmpA	-
FSZ17_RS03235	625107..625580	+	474	WP_057775735.1	SprT family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12955.97 Da
• Isoelectric Point: 4.7051

>T135997 WP_057775733.1 NZ_CP042593:621672-622022 [Bacillus dafuensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITSLDDEMMEKVDEAVQISLGLIEF

Nucleotide

• Length: 351 bp

>T135997 NZ_CP042593:621672-622022 [Bacillus dafuensis]
TTGATTGTCAAACGTGGTGACGTTTATTTTGCAGACCTATCCCCAGTTGTTGGTTCAGAACAAGGTGGCGTCCGTCCAGT
GCTTGTCATCCAAAACGACATCGGGAATCGGTTTAGTCCCACAGTAATTGTTGCAGCAATCACTGCTCAAATTCAAAAAG
CTAAGCTGCCTACTCATGTTGAAATTGATGCAAAGCGCTACGGCTTTGAAAGGGATTCAGTCATCTTGTTAGAGCAGATT
CGAACAATCGATAAACAGCGCTTAACCGATAAGATAACTTCTCTCGATGACGAAATGATGGAAAAAGTGGATGAAGCCGT
ACAAATTAGCTTAGGTCTCATCGAATTTTAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10798.39 Da
• Isoelectric Point: 7.2038

>AT135997 WP_057775732.1 NZ_CP042593:621386-621667 [Bacillus dafuensis]
VSESSATTEIMVKLPQHLLTELDGFVKQENVNRSEFIYQATKMYLRERKKRQIRESMRRGYMEMAKINLTIASEAFLAEF
EAEHTVERLVSGG

Nucleotide

• Length: 282 bp

>AT135997 NZ_CP042593:621386-621667 [Bacillus dafuensis]
GTGTCTGAGTCCAGCGCAACAACAGAGATAATGGTTAAATTACCGCAACATCTTTTAACCGAGTTAGATGGCTTTGTTAA
GCAGGAAAACGTAAATCGGAGTGAATTCATTTATCAAGCAACCAAAATGTATTTGCGCGAACGAAAAAAAAGACAAATTC
GTGAATCCATGAGACGCGGCTATATGGAAATGGCGAAAATAAACTTGACGATTGCATCTGAAGCATTTCTAGCAGAATTC
GAGGCGGAACACACAGTTGAACGTCTAGTAAGCGGAGGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A5B8Z0R5