Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-DnaT
Location 30397..30919 Replicon plasmid pRHEcCUB-1
Accession NZ_CP042589
Organism Escherichia coli strain PK6

Toxin (Protein)


Gene name relE Uniprot ID G3CAN5
Locus tag FTO72_RS23285 Protein ID WP_000220560.1
Coordinates 30638..30919 (+) Length 94 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID G3CAG1
Locus tag FTO72_RS23280 Protein ID WP_000121743.1
Coordinates 30397..30648 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FTO72_RS23245 26068..26349 + 282 WP_001185482.1 hypothetical protein -
FTO72_RS23250 26374..27351 + 978 WP_024245156.1 hypothetical protein -
FTO72_RS23255 27348..27773 - 426 WP_000202942.1 hypothetical protein -
FTO72_RS23260 27841..28287 - 447 Protein_34 hypothetical protein -
FTO72_RS23265 28327..29163 - 837 WP_001050931.1 replication initiation protein -
FTO72_RS23280 30397..30648 + 252 WP_000121743.1 plasmid stabilization protein Antitoxin
FTO72_RS23285 30638..30919 + 282 WP_000220560.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
FTO72_RS23290 30994..31691 + 698 Protein_38 IS1 family transposase -
FTO72_RS23295 31732..32046 + 315 WP_000734776.1 IncN plasmid KikA protein -
FTO72_RS23300 32082..32393 + 312 WP_001452736.1 hypothetical protein -
FTO72_RS23305 32602..33072 + 471 WP_094320909.1 restriction endonuclease -
FTO72_RS23310 33094..34062 + 969 WP_000654802.1 IS5-like element IS903B family transposase -
FTO72_RS23315 34161..34358 + 198 WP_000844102.1 hypothetical protein -
FTO72_RS23320 34378..34650 - 273 Protein_44 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid sul3 / ant(3'')-Ia / cmlA1 / aadA2 / dfrA12 / blaCTX-M-32 - 1..42683 42683


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 11005.83 Da        Isoelectric Point: 10.5938

>T135996 WP_000220560.1 NZ_CP042589:30638-30919 [Escherichia coli]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADLR

Download         Length: 282 bp

>T135996 NZ_CP042589:30638-30919 [Escherichia coli]
ATGACCTATGAACTGGCTTTTGACCGTAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAACTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGCTACAGACTTGTATACCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCCGTTGGAAGA
AGGGAAAGCAGCGAAGTCTATCAGATCGCAGATTTGCGCTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9194.43 Da        Isoelectric Point: 4.1683

>AT135996 WP_000121743.1 NZ_CP042589:30397-30648 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Download         Length: 252 bp

>AT135996 NZ_CP042589:30397-30648 [Escherichia coli]
ATGAGCTATCAAATTCTGACAACTACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G3CAN5

Antitoxin

Source ID Structure
AlphaFold DB A0A3S5I301

References