Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2489394..2490032 Replicon chromosome
Accession NZ_CP042588
Organism Escherichia coli strain PK6

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag FTO72_RS12315 Protein ID WP_000813794.1
Coordinates 2489856..2490032 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag FTO72_RS12310 Protein ID WP_001270286.1
Coordinates 2489394..2489810 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FTO72_RS12290 2484546..2485487 - 942 WP_001251304.1 ABC transporter permease -
FTO72_RS12295 2485488..2486501 - 1014 WP_000220396.1 ABC transporter ATP-binding protein -
FTO72_RS12300 2486519..2487664 - 1146 WP_000047424.1 ABC transporter substrate-binding protein -
FTO72_RS12305 2487909..2489315 - 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
FTO72_RS12310 2489394..2489810 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
FTO72_RS12315 2489856..2490032 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
FTO72_RS12320 2490254..2490484 + 231 WP_000494244.1 YncJ family protein -
FTO72_RS12325 2490576..2492537 - 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
FTO72_RS12330 2492610..2493146 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
FTO72_RS12335 2493199..2494413 + 1215 WP_001326689.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T135992 WP_000813794.1 NZ_CP042588:c2490032-2489856 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T135992 NZ_CP042588:c2490032-2489856 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT135992 WP_001270286.1 NZ_CP042588:c2489810-2489394 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT135992 NZ_CP042588:c2489810-2489394 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References