Detailed information of TA system TA135966

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4046316..4047148
• Replicon: chromosome
• Accession: NZ_CP042585
• Organism: Escherichia coli strain LD91-1

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A836ZBT7
• Locus tag: FQR64_RS20025
• Protein ID: WP_001598217.1
• Coordinates: 4046316..4046690 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: FQR64_RS20030
• Protein ID: WP_001598216.1
• Coordinates: 4046780..4047148 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FQR64_RS19990	4041670..4042650	+	981	WP_001366032.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
FQR64_RS19995	4042658..4042780	-	123	WP_001338066.1	hypothetical protein	-
FQR64_RS20000	4042862..4043734	+	873	WP_000168567.1	HNH endonuclease	-
FQR64_RS20005	4043845..4044843	-	999	WP_001240355.1	membrane protein	-
FQR64_RS25695	4045382..4045534	-	153	WP_001598220.1	hypothetical protein	-
FQR64_RS20015	4045619..4045816	-	198	WP_024192776.1	DUF957 domain-containing protein	-
FQR64_RS20020	4045828..4046319	-	492	WP_001598218.1	hypothetical protein	-
FQR64_RS20025	4046316..4046690	-	375	WP_001598217.1	TA system toxin CbtA family protein	Toxin
FQR64_RS20030	4046780..4047148	-	369	WP_001598216.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
FQR64_RS20035	4047228..4047449	-	222	WP_146881475.1	DUF987 domain-containing protein	-
FQR64_RS20040	4047518..4047994	-	477	WP_001186725.1	RadC family protein	-
FQR64_RS20045	4048009..4048494	-	486	WP_146881476.1	antirestriction protein	-
FQR64_RS20050	4048587..4049405	-	819	WP_146881477.1	DUF945 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimB	4037706..4080582	42876

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13990.98 Da
• Isoelectric Point: 7.8045

>T135966 WP_001598217.1 NZ_CP042585:c4046690-4046316 [Escherichia coli]
MKTLPDTHVREASRCPSPVTIWQTLLGRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAK

Nucleotide

• Length: 375 bp

>T135966 NZ_CP042585:c4046690-4046316 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTTGG
CCGTCTGCTGGACCAGCACTACGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGTATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATAACCCTGGGTAAGCATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13605.40 Da
• Isoelectric Point: 7.3992

>AT135966 WP_001598216.1 NZ_CP042585:c4047148-4046780 [Escherichia coli]
VSDTLSGTTHPDDNNSHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSRGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT135966 NZ_CP042585:c4047148-4046780 [Escherichia coli]
GTGTCAGACACACTCTCCGGGACAACTCATCCCGATGACAACAACAGCCACCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTACTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAGCTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTTACCTGCAAAGCCGATACCCTCGGCAGTCG
AGGTTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A836ZBT7