Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 142267..142793 Replicon plasmid pC16_001
Accession NZ_CP042579
Organism Enterobacter kobei strain C16

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag FR825_RS25150 Protein ID WP_000323025.1
Coordinates 142506..142793 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag FR825_RS25145 Protein ID WP_000534858.1
Coordinates 142267..142506 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FR825_RS25125 138595..139833 + 1239 WP_000219087.1 IS110 family transposase -
FR825_RS25130 140309..140881 + 573 WP_001515348.1 cytochrome b/b6 domain-containing protein -
FR825_RS25135 141081..142004 + 924 WP_000167917.1 cation diffusion facilitator family transporter -
FR825_RS25145 142267..142506 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
FR825_RS25150 142506..142793 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
FR825_RS25155 142865..143023 + 159 WP_001447866.1 type I toxin-antitoxin system Hok family toxin -
FR825_RS25160 143632..143952 - 321 WP_000332796.1 hypothetical protein -
FR825_RS25165 144234..144437 - 204 WP_001015183.1 hypothetical protein -
FR825_RS25170 144484..144834 - 351 WP_000743059.1 hypothetical protein -
FR825_RS25175 144894..145496 - 603 WP_012695474.1 hypothetical protein -
FR825_RS25180 145592..146536 - 945 WP_000778029.1 DUF5417 domain-containing protein -
FR825_RS26925 147047..147223 + 177 WP_001371930.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaTEM-1B / tet(D) / mcr-9 / sul2 / blaSHV-12 / catA2 - 1..275807 275807


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T135946 WP_000323025.1 NZ_CP042579:142506-142793 [Enterobacter kobei]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T135946 NZ_CP042579:142506-142793 [Enterobacter kobei]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT135946 WP_000534858.1 NZ_CP042579:142267-142506 [Enterobacter kobei]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT135946 NZ_CP042579:142267-142506 [Enterobacter kobei]
ATGGGTAGCATTAACCTACGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGCGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGACTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References