Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 95366..96989 Replicon plasmid pC16_001
Accession NZ_CP042579
Organism Enterobacter kobei strain C16

Toxin (Protein)


Gene name hipA Uniprot ID C1IUI3
Locus tag FR825_RS24845 Protein ID WP_000088044.1
Coordinates 95664..96989 (+) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A6M3PBR7
Locus tag FR825_RS24840 Protein ID WP_012477377.1
Coordinates 95366..95659 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FR825_RS24815 91039..92186 + 1148 WP_085949497.1 IS3 family transposase -
FR825_RS24820 92216..92872 + 657 WP_000462754.1 trypsin-like serine protease -
FR825_RS24830 93075..93572 + 498 WP_000340139.1 membrane protein -
FR825_RS24835 93577..94965 + 1389 WP_012695445.1 hypothetical protein -
FR825_RS24840 95366..95659 + 294 WP_012477377.1 helix-turn-helix transcriptional regulator Antitoxin
FR825_RS24845 95664..96989 + 1326 WP_000088044.1 type II toxin-antitoxin system HipA family toxin Toxin
FR825_RS24850 97050..97256 + 207 WP_000134171.1 hypothetical protein -
FR825_RS24855 97357..97767 + 411 WP_000985909.1 hypothetical protein -
FR825_RS24860 97780..98208 + 429 Protein_94 hypothetical protein -
FR825_RS24865 98486..99490 - 1005 WP_000427623.1 IS110-like element IS4321 family transposase -
FR825_RS24870 99581..100015 - 435 WP_000405672.1 Cd(II)/Pb(II)-responsive transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaTEM-1B / tet(D) / mcr-9 / sul2 / blaSHV-12 / catA2 - 1..275807 275807
- flank IS/Tn - - 98486..99490 1004


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(8-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50333.32 Da        Isoelectric Point: 6.5044

>T135945 WP_000088044.1 NZ_CP042579:95664-96989 [Enterobacter kobei]
MSRKQQRLAIWMNGIKVGYWEKSKGVDSLQYLPDWSADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMRSSVENEWLCSVLLEQYGLPVARTQIAHFEDQKALVVERF
DRKWSGNGQWIIRLPQEDMCQALGISPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIVFWMMAATDGHAKNFS
ISIGPQGRYHLTPLYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

>T135945 NZ_CP042579:95664-96989 [Enterobacter kobei]
ATGAGCCGGAAACAGCAGCGTCTGGCAATCTGGATGAACGGAATCAAAGTCGGATACTGGGAAAAAAGTAAAGGGGTAGA
CAGCTTACAATACCTTCCTGACTGGAGTGCAGACGAACAGGGAAGGCCGCTGTCATTGTCCCTTCCTTTTACTCCCGGGA
ACCAGGTCTGGCGTGGAAATGTGGTACGTGACTATTTTGATAATTTACTCCCGGACAGCGAAGGCATACGCAGACGTCTG
GCAATGCGTTACAAGGCCGATAGCCTGGAGCCTTTTGATCTTCTGACGGAGCTGGGAAAAGACTGCGTGGGCGCGATACA
GCTGCTTCATGACGGAGATGAACCCACAGATTTATATTCCGTGAAGTATCACCCGCTTACTGAATCAGAAATTGCGGCAA
CTTTGCGTAATACCACGGAGACATTGCTACCGGGCAGGCCAGAAGATAACGACGACTTACGTTTATCTATTGCCGGTGCT
CAGGAGAAAACAGCCCTACTGTGGCATGAAGATCGGTGGTGTATGCCGGAGGGTAATACACCAACGACGCATATATTCAA
GCTACCGCTCGGACTTGTGGGGAACATGAAAGCCGACATGCGCTCGTCGGTTGAAAATGAATGGCTCTGCTCGGTACTTC
TCGAACAGTACGGGCTCCCCGTGGCAAGAACGCAGATTGCTCACTTTGAAGATCAGAAAGCGCTGGTGGTAGAACGTTTT
GACCGAAAATGGTCAGGAAACGGACAATGGATCATTCGTTTACCCCAGGAGGATATGTGTCAGGCCCTGGGCATTTCGCC
ATTACGAAAATATCAGGCGGACGGCGGGCCAGGTATATCGGAAATTATGGAAGTTCTGAGTAATTCAGACCGTGCTGAGC
GTGACAAAGCGCAGTTTTTTATGACGCAGATAGTTTTCTGGATGATGGCCGCAACAGATGGCCACGCTAAAAATTTCAGT
ATTTCTATTGGGCCACAGGGACGTTATCACCTTACACCACTCTATGATGTGTTGTCGGCATGGCCGGTGATCGGTCATGG
GAACAATCAGATATCCTGGCAGAAGTGCAAACTGGCGATGGCCGTACGTGGCAGTAGCAACTATTACCAGATATATCGAA
TACAACGACGCCACTGGATCAGGCATGGTGAGATAACCGGCCTGAGTAAACAGCAGACAGAGGCGATGATAGAGGAAATC
ATCGCCAGAACCCCGGGTGTCATTGAACGTGTTAGCGGGCTTCTTCCGGACCAGTTCCCCCAGCAGCTTGCGGAAAGTAT
TTTTGATGGAATGAGGCAGCAATGCAGGCGCCTGGCTGAGAAATGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10860.30 Da        Isoelectric Point: 10.3134

>AT135945 WP_012477377.1 NZ_CP042579:95366-95659 [Enterobacter kobei]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSTTASSEGK
QTEEMLKSNSPARQEKW

Download         Length: 294 bp

>AT135945 NZ_CP042579:95366-95659 [Enterobacter kobei]
ATCAATAATGATTACCCGTTAAACACGCTCAACCAGTTGCGTCCCTTGCTCATTGGTTTTCGCAAGGCGAATGGCCTGAC
GCAAAAAGACCTGTCCGAAAGGTTAGGGGTCACTCAACAGACATACTCACGTCTGGAAGCCAACCCTGCCAGTGCGAGCA
TTGAACGGCTATTCAAGGTGTTTTCTATCCTGGGCGTAAAAATCAGCTTCTCCTCGACAACCGCCTCTTCAGAGGGGAAG
CAGACGGAAGAAATGCTTAAATCAAACTCACCTGCACGACAGGAGAAATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2S1XXW1

Antitoxin

Source ID Structure
AlphaFold DB A0A6M3PBR7

References