Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/FR47-DUF1778
Location 42141..42938 Replicon plasmid pE5_002
Accession NZ_CP042573
Organism Enterobacter hormaechei strain E5

Toxin (Protein)


Gene name KacT Uniprot ID F5S3R9
Locus tag FR760_RS24620 Protein ID WP_006812498.1
Coordinates 42414..42938 (+) Length 175 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID F5S3R8
Locus tag FR760_RS24615 Protein ID WP_006812497.1
Coordinates 42141..42410 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FR760_RS24590 37521..38861 - 1341 WP_121909366.1 AAA family ATPase -
FR760_RS24595 38922..39647 - 726 WP_151884546.1 hypothetical protein -
FR760_RS24600 39911..40708 + 798 WP_006812584.1 DUF2971 domain-containing protein -
FR760_RS24605 40750..41103 - 354 WP_160859104.1 hypothetical protein -
FR760_RS24610 41109..41774 - 666 WP_023315962.1 AAA family ATPase -
FR760_RS24615 42141..42410 + 270 WP_006812497.1 DUF1778 domain-containing protein Antitoxin
FR760_RS24620 42414..42938 + 525 WP_006812498.1 GNAT family N-acetyltransferase Toxin
FR760_RS25865 42965..43171 - 207 WP_023316023.1 hypothetical protein -
FR760_RS25870 43122..43373 - 252 WP_000147960.1 hypothetical protein -
FR760_RS24630 43375..44067 - 693 WP_006812500.1 membrane protein -
FR760_RS24635 44081..44404 - 324 WP_000064173.1 hypothetical protein -
FR760_RS24640 44479..45267 - 789 WP_032655902.1 receptor-recognizing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..114054 114054


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-149)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 175 a.a.        Molecular weight: 19356.13 Da        Isoelectric Point: 8.9844

>T135930 WP_006812498.1 NZ_CP042573:42414-42938 [Enterobacter hormaechei]
VANLTIEMFSEEAVYDFSCFDCGETSLNDFLNNRLAQQHSGRILRGYLLLTRDPIPKVMGFYTLSGSCFARNTLPSNTQQ
RKVPYSDAPSVTLGRLAIDKSIQRQGYGETLVAHAMKVVYRASLAVGIYALFVDAKNENAMRFYKQLGFTPLTGDNANSL
FYPTKGIEKLFGGK

Download         Length: 525 bp

>T135930 NZ_CP042573:42414-42938 [Enterobacter hormaechei]
GTGGCCAACCTGACCATTGAGATGTTTTCAGAAGAGGCTGTATATGATTTCTCATGCTTCGACTGCGGAGAAACGTCTCT
CAATGATTTTCTTAACAATCGCCTCGCCCAGCAACACAGCGGGCGTATATTACGCGGATATCTGCTTCTGACGAGAGATC
CGATACCGAAAGTGATGGGGTTTTACACGTTGTCCGGGAGCTGCTTTGCAAGAAACACGCTTCCTTCCAACACTCAACAG
CGTAAAGTCCCCTATTCCGACGCCCCAAGCGTTACGCTCGGTCGTCTGGCAATAGACAAAAGTATCCAGCGACAAGGGTA
TGGGGAAACGCTGGTGGCACATGCGATGAAAGTGGTATACCGGGCTTCCCTGGCCGTTGGGATCTACGCTTTGTTTGTTG
ACGCCAAAAATGAGAATGCGATGCGGTTTTATAAGCAGTTAGGCTTTACCCCTCTTACGGGTGATAACGCCAATTCGCTG
TTCTATCCCACAAAAGGCATTGAGAAGTTGTTTGGTGGAAAATAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10114.51 Da        Isoelectric Point: 4.8483

>AT135930 WP_006812497.1 NZ_CP042573:42141-42410 [Enterobacter hormaechei]
MSALKKQRIDLRLTDDDKSMIEEAAAMTNQTITQFMVASASERAAEVIEQHRRLILSEESWNIVMDAISNPPAPNDRLKR
AAERLQSME

Download         Length: 270 bp

>AT135930 NZ_CP042573:42141-42410 [Enterobacter hormaechei]
ATGTCAGCACTGAAAAAACAGCGCATCGATCTCAGATTAACCGACGACGACAAGAGCATGATCGAAGAAGCTGCGGCAAT
GACCAACCAGACAATTACTCAGTTTATGGTTGCCAGTGCCTCTGAACGTGCTGCGGAAGTGATAGAGCAACATCGTCGCC
TGATTCTTAGCGAAGAGTCATGGAATATAGTGATGGATGCCATCAGTAACCCGCCTGCACCGAATGATAGGCTGAAACGA
GCTGCCGAGCGTCTACAAAGCATGGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J0QWY8

Antitoxin

Source ID Structure
AlphaFold DB A0A2J0QUA3

References