Detailed information of TA system TA135929

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): stbDE/ParE-YafN
• Location: 152493..153015
• Replicon: plasmid pE5_001
• Accession: NZ_CP042572
• Organism: Enterobacter hormaechei strain E5

Toxin (Protein)

• Gene name: stbE
• Uniprot ID: A0A9J6S4Z8
• Locus tag: FR760_RS24275
• Protein ID: WP_004187019.1
• Coordinates: 152731..153015 (+)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: stbD
• Uniprot ID: A0A2J4ZSR4
• Locus tag: FR760_RS24270
• Protein ID: WP_004187025.1
• Coordinates: 152493..152741 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FR760_RS24245	147602..147760	-	159	WP_151884524.1	type I toxin-antitoxin system Hok family toxin	-
FR760_RS24250	147915..148706	-	792	WP_151884525.1	sulfite exporter TauE/SafE family protein	-
FR760_RS24255	148816..149583	+	768	WP_151884526.1	helix-turn-helix transcriptional regulator	-
FR760_RS24260	149680..150576	-	897	WP_192797759.1	LysR family transcriptional regulator	-
FR760_RS24265	150688..151851	+	1164	WP_151884527.1	alpha/beta fold hydrolase	-
FR760_RS24270	152493..152741	+	249	WP_004187025.1	plasmid stabilization protein	Antitoxin
FR760_RS24275	152731..153015	+	285	WP_004187019.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FR760_RS24280	153037..154374	-	1338	Protein_153	ISNCY family transposase	-
FR760_RS24285	154923..155438	+	516	WP_151884528.1	hypothetical protein	-
FR760_RS24290	156593..157453	-	861	WP_151884529.1	SMP-30/gluconolactonase/LRE family protein	-
FR760_RS24295	157531..158004	-	474	WP_151884530.1	DMT family transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..167131	167131

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-84)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 11003.72 Da
• Isoelectric Point: 10.5388

>T135929 WP_004187019.1 NZ_CP042572:152731-153015 [Enterobacter hormaechei]
MTYKLAFNESALKEWKKLGHTLQVQFKKKLKERLENPRVPASQLHGRKDQYKIKLRGAGYRLVYRVEDDIVTVTVIGVGK
RENDDIYNATLNRN

Nucleotide

• Length: 285 bp

>T135929 NZ_CP042572:152731-153015 [Enterobacter hormaechei]
ATGACCTATAAGCTGGCTTTCAACGAATCCGCTCTGAAAGAATGGAAGAAGCTGGGTCATACACTGCAGGTACAGTTCAA
AAAGAAGCTAAAAGAACGCCTGGAGAACCCAAGAGTTCCGGCGTCACAGCTACATGGACGTAAGGACCAGTATAAAATTA
AGTTACGTGGTGCCGGATATCGCCTTGTCTACCGTGTAGAGGACGACATTGTCACAGTCACTGTTATCGGCGTAGGCAAA
CGCGAAAACGACGACATTTATAATGCAACCCTTAATCGCAATTGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9011.24 Da
• Isoelectric Point: 3.9947

>AT135929 WP_004187025.1 NZ_CP042572:152493-152741 [Enterobacter hormaechei]
MAFQVLTTTAASITELKRDPMGTFHAGDGAPVAILNRNEPAFYCVPPALYAQLMELLEDEELGRMADERSGEKVIEVNID
DL

Nucleotide

• Length: 249 bp

>AT135929 NZ_CP042572:152493-152741 [Enterobacter hormaechei]
ATGGCATTTCAGGTTCTGACCACTACGGCAGCCAGCATTACTGAGCTTAAACGTGACCCTATGGGTACTTTCCACGCCGG
GGATGGCGCTCCGGTAGCCATTCTTAATCGTAATGAGCCTGCATTCTACTGTGTGCCACCGGCACTGTATGCCCAGTTGA
TGGAGCTGCTGGAAGATGAAGAACTGGGGAGGATGGCTGATGAGCGAAGCGGAGAAAAGGTTATAGAGGTCAATATTGAT
GACCTATAA

Structures

Antitoxin

• AlphaFold DB: A0A2J4ZSR4