Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/ElaA-DUF1778
Location 69867..70606 Replicon plasmid pC44_001
Accession NZ_CP042567
Organism Enterobacter hormaechei strain C44

Toxin (Protein)


Gene name tacT Uniprot ID A0A5E1AVR5
Locus tag FR837_RS23035 Protein ID WP_013087172.1
Coordinates 70121..70606 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID A0A5E1AWX8
Locus tag FR837_RS23030 Protein ID WP_012540086.1
Coordinates 69867..70133 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FR837_RS23000 65728..67494 - 1767 Protein_68 Tn3 family transposase -
FR837_RS23010 67546..68250 - 705 WP_001067855.1 IS6-like element IS26 family transposase -
FR837_RS23015 68304..68519 - 216 Protein_70 RNA polymerase subunit sigma-70 -
FR837_RS23020 68528..69241 - 714 WP_013087170.1 arsenical resistance protein ArsH -
FR837_RS23025 69243..69566 - 324 WP_013087171.1 metalloregulator ArsR/SmtB family transcription factor -
FR837_RS23030 69867..70133 + 267 WP_012540086.1 DUF1778 domain-containing protein Antitoxin
FR837_RS23035 70121..70606 + 486 WP_013087172.1 GNAT family N-acetyltransferase Toxin
FR837_RS23040 71025..72365 + 1341 WP_000589001.1 ISNCY family transposase -
FR837_RS23045 72621..73043 - 423 WP_032072095.1 nickel resistance OB fold protein NcrY -
FR837_RS23050 73205..74335 - 1131 WP_004196366.1 Ni(II)/Co(II) efflux transporter permease subunit NcrC -
FR837_RS23055 74348..74617 - 270 WP_004196355.1 nickel-sensing transcriptional repressor NcrB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid dfrA14 / blaTEM-1B / aph(6)-Id / aph(3'')-Ib / sul2 - 1..150626 150626


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(27-152)

Antitoxin

Vibrio phage ICP1, Orf50

(2-87)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17810.62 Da        Isoelectric Point: 9.8560

>T135913 WP_013087172.1 NZ_CP042567:70121-70606 [Enterobacter hormaechei]
VGRVTVPEPLSSFHQVAEFVCGETVLDDWLKQKGLKNQALGSARTFVVCKKDTKQVAGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDLSFRGKGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKNFYIHHGFKPSQTQQRTLFLKLP
Q

Download         Length: 486 bp

>T135913 NZ_CP042567:70121-70606 [Enterobacter hormaechei]
GTGGGACGTGTAACAGTACCAGAACCTTTATCCAGCTTTCATCAGGTGGCGGAATTCGTTTGCGGTGAAACTGTTCTGGA
TGACTGGTTAAAGCAGAAAGGCCTCAAAAACCAGGCTCTCGGATCGGCCAGAACGTTTGTAGTGTGTAAAAAAGACACGA
AACAAGTAGCCGGTTTTTACTCTCTGGCCACCGGGAGCGTCAACCATACAGAAGCGACAGGCAATCTTCGGCGTAACATG
CCCGATCCCATCCCTGTTATTATACTTGCCCGTCTTGCTGTCGATCTCTCTTTCCGCGGAAAAGGGCTTGGCGCTGATTT
ACTTCATGATGCAGTGCTTCGTTGCTATCGGGTTGCCGAGAATATTGGTGTACGTGCAATCATGGTTCATGCACTCACCG
AAGAAGCTAAAAATTTCTACATTCACCATGGTTTCAAACCATCACAAACTCAGCAACGGACATTATTTCTTAAACTCCCT
CAATAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10076.47 Da        Isoelectric Point: 4.3721

>AT135913 WP_012540086.1 NZ_CP042567:69867-70133 [Enterobacter hormaechei]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVADDPAIDKLL
ARKPQWDV

Download         Length: 267 bp

>AT135913 NZ_CP042567:69867-70133 [Enterobacter hormaechei]
ATGAAATCAGATGTTCAACTTAATCTTAGAGCAAAGGAATCACAGCGGGCGCTCATTGATGCTGCTGCGGAAATTCTTCA
CAAATCACGAACAGACTTCATCCTGGAAATGGCCTGTCAGGCCGCTGAGAACGTGATCCTCGATCGCCGGGTCTTTAATT
TTAACGATGAACAATATGCCGAATTCATCGACATGCTTGATGCCCCTGTTGCAGATGATCCTGCCATAGATAAACTGCTG
GCAAGGAAACCTCAGTGGGACGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5E1AVR5

Antitoxin

Source ID Structure
AlphaFold DB A0A5E1AWX8

References