Detailed information of TA system TA135894

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 14012..14596
• Replicon: plasmid pE47_001
• Accession: NZ_CP042557
• Organism: Acinetobacter baumannii strain E47

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: N8Q7V3
• Locus tag: FR761_RS18660
• Protein ID: WP_000286964.1
• Coordinates: 14012..14314 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: FR761_RS18665
• Protein ID: WP_000985609.1
• Coordinates: 14315..14596 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FR761_RS18630	9458..10285	+	828	WP_000073658.1	AraC family transcriptional regulator	-
FR761_RS18635	10335..10940	+	606	WP_000447788.1	LysE family translocator	-
FR761_RS18640	11060..12394	+	1335	WP_010326716.1	ISNCY family transposase	-
FR761_RS18645	12656..12943	+	288	WP_008942539.1	BrnT family toxin	-
FR761_RS18650	12930..13244	+	315	WP_151532470.1	BrnA antitoxin family protein	-
FR761_RS18655	13333..13863	-	531	WP_000172759.1	hypothetical protein	-
FR761_RS18660	14012..14314	+	303	WP_000286964.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FR761_RS18665	14315..14596	+	282	WP_000985609.1	putative addiction module antidote protein	Antitoxin
FR761_RS18670	15089..16564	+	1476	WP_151532471.1	ABC-F type ribosomal protection protein Msr(E)	-
FR761_RS18675	16620..17504	+	885	WP_000155092.1	Mph(E) family macrolide 2'-phosphotransferase	-
FR761_RS18685	17757..19082	+	1326	WP_008942541.1	ISNCY family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaOXA-96 / msr(E) / mph(E) / aac(3)-IId / cmlB1 / sul1 / qacE / catB3 / blaIMP-4 / dfrA19 / blaCARB-2 / aph(3')-Ia	-	1..327867	327867
	-	inside	IScluster/Tn	blaOXA-96 / msr(E) / mph(E)	-	3514..22101	18587

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-90)

Antitoxin

• Uncharacterized protein HI1420 (4-91)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11573.07 Da
• Isoelectric Point: 4.6838

>T135894 WP_000286964.1 NZ_CP042557:14012-14314 [Acinetobacter baumannii]
MYSIYTTEVFDDWFTKLKDQQAKRRIQVRIDRVEDGNFGDTEPVGEGVSELRFFFGPGYRIYYCKQGQRVVILLAGGDKS
TQSKDIKLALQLAQDLEEEL

Nucleotide

• Length: 303 bp

>T135894 NZ_CP042557:14012-14314 [Acinetobacter baumannii]
ATGTACTCAATATATACCACTGAAGTCTTTGATGACTGGTTCACCAAGTTAAAAGACCAGCAGGCAAAAAGACGCATACA
AGTCCGAATTGATCGGGTTGAAGATGGAAACTTTGGAGATACTGAGCCGGTCGGTGAAGGTGTTTCTGAATTACGTTTCT
TCTTTGGGCCAGGCTATAGGATTTATTACTGCAAGCAAGGGCAAAGGGTTGTTATTCTTTTAGCAGGCGGAGATAAGTCT
ACGCAAAGTAAAGATATAAAACTTGCCCTGCAATTGGCACAAGATTTAGAGGAGGAGCTATAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10109.67 Da
• Isoelectric Point: 8.0018

>AT135894 WP_000985609.1 NZ_CP042557:14315-14596 [Acinetobacter baumannii]
MAIKLRKWDSAEHLKTEDDMQAYLQACIEESNGDAAFIAKALGNIAKAKGMAQLSRDTGLGRESLYKALSGDVNPSFDTV
IKVVKALNLRLAI

Nucleotide

• Length: 282 bp

>AT135894 NZ_CP042557:14315-14596 [Acinetobacter baumannii]
ATGGCTATTAAACTTCGCAAATGGGACAGTGCTGAGCATCTTAAAACAGAGGATGATATGCAGGCTTACTTACAAGCATG
TATTGAAGAATCTAATGGCGATGCCGCATTCATTGCAAAGGCTTTAGGTAACATTGCAAAAGCTAAAGGAATGGCTCAAT
TATCACGAGATACAGGCTTAGGCCGAGAAAGTCTTTATAAAGCACTTTCTGGTGATGTTAACCCTAGTTTTGATACTGTG
ATTAAAGTTGTAAAAGCACTGAATTTACGCTTAGCAATTTAA

Structures

Toxin

• AlphaFold DB: A0A0A8TV94