Detailed information of TA system TA135893

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 12656..13244
• Replicon: plasmid pE47_001
• Accession: NZ_CP042557
• Organism: Acinetobacter baumannii strain E47

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: C0VLQ9
• Locus tag: FR761_RS18645
• Protein ID: WP_008942539.1
• Coordinates: 12656..12943 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: FR761_RS18650
• Protein ID: WP_151532470.1
• Coordinates: 12930..13244 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FR761_RS18625	8578..9330	+	753	Protein_10	IS1-like element ISAba3 family transposase	-
FR761_RS18630	9458..10285	+	828	WP_000073658.1	AraC family transcriptional regulator	-
FR761_RS18635	10335..10940	+	606	WP_000447788.1	LysE family translocator	-
FR761_RS18640	11060..12394	+	1335	WP_010326716.1	ISNCY family transposase	-
FR761_RS18645	12656..12943	+	288	WP_008942539.1	BrnT family toxin	Toxin
FR761_RS18650	12930..13244	+	315	WP_151532470.1	BrnA antitoxin family protein	Antitoxin
FR761_RS18655	13333..13863	-	531	WP_000172759.1	hypothetical protein	-
FR761_RS18660	14012..14314	+	303	WP_000286964.1	type II toxin-antitoxin system RelE/ParE family toxin	-
FR761_RS18665	14315..14596	+	282	WP_000985609.1	putative addiction module antidote protein	-
FR761_RS18670	15089..16564	+	1476	WP_151532471.1	ABC-F type ribosomal protection protein Msr(E)	-
FR761_RS18675	16620..17504	+	885	WP_000155092.1	Mph(E) family macrolide 2'-phosphotransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaOXA-96 / msr(E) / mph(E) / aac(3)-IId / cmlB1 / sul1 / qacE / catB3 / blaIMP-4 / dfrA19 / blaCARB-2 / aph(3')-Ia	-	1..327867	327867
	-	inside	IScluster/Tn	blaOXA-96 / msr(E) / mph(E)	-	3514..22101	18587

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (41-96)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11333.70 Da
• Isoelectric Point: 5.2216

>T135893 WP_008942539.1 NZ_CP042557:12656-12943 [Acinetobacter baumannii]
MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTDGEERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERDRYEHG

Nucleotide

• Length: 288 bp

>T135893 NZ_CP042557:12656-12943 [Acinetobacter baumannii]
ATGGAACAGTATTTTGAATGGGATGAGGCTAAGAATCGAAAGAATCAGAAAAAGCATGATATCTCTTTCGAAACTGCAAG
CCTTGTTTTTGAAGATCCATTACGGATCTCAATCCAAGACAGACATACCGATGGTGAAGAACGTTGGCAAACCATTGGAC
GAGTTAAAGGCGTACTAATGCTCTTAGTAGCTCACACCATCTTTGATGAAGATGACTGTGAAATCATACGAATCATTAGT
GCAAGACAAGTAACTAAGGCGGAGCGAGATAGATATGAGCATGGTTAG

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12218.89 Da
• Isoelectric Point: 8.4628

>AT135893 WP_151532470.1 NZ_CP042557:12930-13244 [Acinetobacter baumannii]
MSMVRYSRKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDMPEITDWSSAVRQNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKNHQ

Nucleotide

• Length: 315 bp

>AT135893 NZ_CP042557:12930-13244 [Acinetobacter baumannii]
ATGAGCATGGTTAGATACTCACGCAAAGAACTGAATGAAAAATTTGGTGAGAAGCAAGATGCTGAAATTCAACGCTTGCT
TGCTAAAGGCACGGTTCCTGACGATCAGCTAGATCTCTCAGATATGCCTGAAATTACCGATTGGAGCAGTGCTGTACGCC
AAAATCAATTTTACCGCCCAGTGAAGCAACAAACTTCAATTCGGTTAGATGCTGATGTACTTGCTTGGTTCAAAGCACAA
GGCAAAGGTTATCAAACACGAATGAATGAAATTTTACGGGATGCTATGCTGAAAGAATTAAAAAATCATCAATAA

Structures

Toxin

• AlphaFold DB: A0A5P6FTP5