Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) kacAT/ElaA-DUF1778
Location 103221..104024 Replicon plasmid pC45_002
Accession NZ_CP042553
Organism Enterobacter hormaechei strain C45

Toxin (Protein)


Gene name KacT Uniprot ID A0A7W3E3C9
Locus tag FR772_RS26955 Protein ID WP_022652313.1
Coordinates 103494..104024 (+) Length 177 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID W8E6Q5
Locus tag FR772_RS26950 Protein ID WP_022652312.1
Coordinates 103221..103490 (+) Length 90 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FR772_RS26930 99526..99833 - 308 Protein_102 IS1 family transposase -
FR772_RS26940 100310..101050 + 741 WP_022652310.1 site-specific integrase -
FR772_RS26945 101376..102365 - 990 WP_022652311.1 RepB family plasmid replication initiator protein -
FR772_RS26950 103221..103490 + 270 WP_022652312.1 DUF1778 domain-containing protein Antitoxin
FR772_RS26955 103494..104024 + 531 WP_022652313.1 GNAT family N-acetyltransferase Toxin
FR772_RS26960 104156..105049 + 894 WP_077873527.1 IS5 family transposase -
FR772_RS26965 105190..106086 - 897 Protein_108 IS3 family transposase -
FR772_RS26970 106151..107302 + 1152 WP_022652315.1 IS30-like element IS30 family transposase -
FR772_RS26975 107411..107794 + 384 WP_001201739.1 IS66 family insertion sequence hypothetical protein -
FR772_RS26980 107791..108138 + 348 WP_000609174.1 IS66 family insertion sequence element accessory protein TnpB -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaIMP-4 / catB3 / blaOXA-10 / ant(3'')-Ia / blaVEB-3 / aac(6')-Ib-cr / blaOXA-1 / ARR-3 / qacE / sul1 / mph(A) / aac(3)-IId - 1..173758 173758
- inside IScluster/Tn - - 98212..111593 13381


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(92-156)

Antitoxin

(8-86)


Sequences


Toxin        


Download         Length: 177 a.a.        Molecular weight: 20349.26 Da        Isoelectric Point: 6.7496

>T135887 WP_022652313.1 NZ_CP042553:103494-104024 [Enterobacter hormaechei]
MEGLRIEIFSEEVEYELSNFDCGEEYLNTFLTDHLKRQHNSKILRGYVLVTRENKPRVMGYYTLSGSCFEKILLPSKTQQ
KRVPYKNVPSVTLGRLAIDKSIHHQGYGETLVTHAMKVVYQASQAVGIHGMFVEALNDNAKKFYLRLGFIQLKEENCNSL
FYPTKSIEELFEVNDE

Download         Length: 531 bp

>T135887 NZ_CP042553:103494-104024 [Enterobacter hormaechei]
ATGGAGGGTTTAAGGATTGAGATTTTTTCAGAGGAAGTTGAATATGAATTAAGCAACTTCGATTGTGGAGAAGAGTATCT
GAATACTTTTCTTACTGACCATCTGAAACGGCAGCATAACAGTAAGATCTTACGTGGTTATGTTCTCGTGACGAGAGAGA
ATAAACCGCGTGTGATGGGGTACTACACGCTATCAGGAAGTTGCTTTGAGAAAATACTTCTACCCAGCAAAACACAACAA
AAGCGTGTACCCTACAAGAATGTGCCGAGCGTGACTTTGGGCCGTCTGGCAATTGATAAAAGCATTCACCACCAGGGATA
TGGAGAAACACTTGTTACCCATGCGATGAAAGTCGTGTATCAGGCCTCGCAGGCTGTGGGTATACACGGAATGTTCGTGG
AAGCGCTGAACGACAACGCCAAAAAATTCTACCTTCGACTGGGTTTCATTCAGTTAAAGGAAGAAAACTGTAACTCGCTC
TTTTACCCGACAAAATCTATTGAAGAATTGTTCGAAGTAAATGATGAATAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10269.70 Da        Isoelectric Point: 5.7252

>AT135887 WP_022652312.1 NZ_CP042553:103221-103490 [Enterobacter hormaechei]
MSALKKQRIDLRLNEDDKHMIEEAAAMTNQSISQFMVSTASERAAEVIDQHRRLLLNEESWNLVMDAIINPPAPNDRLKR
AANRLRELE

Download         Length: 270 bp

>AT135887 NZ_CP042553:103221-103490 [Enterobacter hormaechei]
ATGTCAGCACTGAAAAAGCAGCGCATTGACCTGAGATTAAATGAAGACGACAAACACATGATCGAAGAAGCTGCAGCAAT
GACTAACCAGTCAATTTCGCAGTTCATGGTCAGTACCGCCTCAGAACGCGCTGCAGAAGTTATTGACCAGCACCGTCGTC
TGCTCCTCAATGAGGAGTCTTGGAACCTGGTTATGGATGCAATCATTAATCCGCCAGCACCGAATGACCGGCTGAAACGC
GCCGCTAACCGTCTCCGGGAACTGGAGTAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A7W3E3C9


Antitoxin

Source ID Structure
AlphaFold DB A0A2Z3XDS5

References