Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 199735..200261 Replicon plasmid pC45_001
Accession NZ_CP042552
Organism Enterobacter hormaechei strain C45

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag FR772_RS25800 Protein ID WP_000323025.1
Coordinates 199735..200022 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag FR772_RS25805 Protein ID WP_000534858.1
Coordinates 200022..200261 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FR772_RS27620 195305..195481 - 177 WP_001371930.1 hypothetical protein -
FR772_RS25770 195992..196936 + 945 WP_000778029.1 DUF5417 domain-containing protein -
FR772_RS25775 197032..197634 + 603 WP_012695474.1 hypothetical protein -
FR772_RS25780 197694..198044 + 351 WP_151608595.1 hypothetical protein -
FR772_RS25785 198091..198294 + 204 WP_001015183.1 hypothetical protein -
FR772_RS25790 198576..198896 + 321 WP_000332796.1 hypothetical protein -
FR772_RS25795 199505..199663 - 159 WP_001447866.1 type I toxin-antitoxin system Hok family toxin -
FR772_RS25800 199735..200022 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
FR772_RS25805 200022..200261 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
FR772_RS25815 200524..201447 - 924 WP_000167917.1 cation diffusion facilitator family transporter -
FR772_RS25820 201647..202219 - 573 WP_001515348.1 cytochrome b/b6 domain-containing protein -
FR772_RS25825 202695..203933 - 1239 WP_000219087.1 IS110 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaTEM-1B / catA2 / tet(D) / mcr-9 / aph(6)-Id / aph(3'')-Ib / dfrA19 / sul1 / qnrA1 / qacE / ere(A) / aac(6')-IIc / blaSHV-12 - 1..288659 288659


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T135885 WP_000323025.1 NZ_CP042552:c200022-199735 [Enterobacter hormaechei]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T135885 NZ_CP042552:c200022-199735 [Enterobacter hormaechei]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT135885 WP_000534858.1 NZ_CP042552:c200261-200022 [Enterobacter hormaechei]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT135885 NZ_CP042552:c200261-200022 [Enterobacter hormaechei]
ATGGGTAGCATTAACCTACGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGCGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGACTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References