Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) KacT-ataR/DUF1778(antitoxin)
Location 1866382..1867179 Replicon chromosome
Accession NZ_CP042551
Organism Enterobacter hormaechei strain C45

Toxin (Protein)


Gene name KacT Uniprot ID A0A222RDD1
Locus tag FR772_RS08975 Protein ID WP_032647926.1
Coordinates 1866658..1867179 (+) Length 174 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID A0A331QJY7
Locus tag FR772_RS08970 Protein ID WP_015570876.1
Coordinates 1866382..1866651 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FR772_RS08935 1861922..1862281 + 360 WP_032647921.1 helix-turn-helix transcriptional regulator -
FR772_RS08940 1862291..1862455 - 165 WP_017693668.1 helix-turn-helix transcriptional regulator -
FR772_RS08945 1862608..1863849 + 1242 WP_032647923.1 bifunctional glucose-1-phosphatase/inositol phosphatase -
FR772_RS08950 1863882..1864109 - 228 WP_006809324.1 YccJ family protein -
FR772_RS08955 1864128..1864724 - 597 WP_006809323.1 NAD(P)H:quinone oxidoreductase -
FR772_RS08960 1865116..1865286 + 171 WP_001273664.1 general stress protein -
FR772_RS08965 1865403..1866308 + 906 WP_017693666.1 DMT family transporter -
FR772_RS08970 1866382..1866651 + 270 WP_015570876.1 DUF1778 domain-containing protein Antitoxin
FR772_RS08975 1866658..1867179 + 522 WP_032647926.1 GNAT family N-acetyltransferase Toxin
FR772_RS08980 1867238..1868560 - 1323 WP_032647928.1 pyrimidine utilization transport protein G -
FR772_RS08985 1868582..1869073 - 492 WP_032647931.1 pyrimidine utilization flavin reductase protein F -
FR772_RS08990 1869086..1869676 - 591 WP_032647932.1 malonic semialdehyde reductase -
FR772_RS08995 1869686..1870486 - 801 WP_032647935.1 pyrimidine utilization protein D -
FR772_RS09000 1870494..1870880 - 387 WP_015570870.1 pyrimidine utilization protein C -
FR772_RS09005 1870892..1871581 - 690 WP_151608505.1 pyrimidine utilization protein B -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(87-149)

Antitoxin

Vibrio phage ICP1, Orf50

(2-88)


Sequences

Toxin        


Download         Length: 174 a.a.        Molecular weight: 19451.21 Da        Isoelectric Point: 7.4318

>T135871 WP_032647926.1 NZ_CP042551:1866658-1867179 [Enterobacter hormaechei]
VANLTIEMLSEGTDYDFGDFDCGEPSLNAFLTEHLIRQHGGRILRGYLLKERDHPRVLGYYTLSGSCFEKAMLPSKTQQR
RIPYSNVPSVTLGRLAVHKELQGNEWGTTLVTHAMRVVYLASQAVGVHGIFVDALNERAKRFYLKLGFIPLAGENSSSLF
FPTQSIERLFEQA

Download         Length: 522 bp

>T135871 NZ_CP042551:1866658-1867179 [Enterobacter hormaechei]
GTGGCAAATCTCACGATCGAAATGTTATCAGAGGGAACCGATTACGATTTCGGCGATTTCGATTGTGGTGAACCCTCGCT
AAATGCCTTCCTTACCGAGCACCTGATACGTCAGCACGGCGGGCGTATTTTACGCGGCTACCTGCTTAAAGAACGCGACC
ATCCGCGTGTCCTGGGCTATTACACACTCTCGGGAAGTTGCTTTGAAAAAGCGATGCTTCCTTCAAAGACTCAGCAGCGC
CGCATCCCATACAGCAACGTCCCCAGCGTGACGCTGGGGCGTCTGGCTGTCCATAAAGAACTCCAGGGAAACGAGTGGGG
AACAACGCTTGTCACACACGCTATGCGCGTTGTTTATCTGGCCTCGCAGGCTGTTGGCGTCCACGGAATTTTTGTGGATG
CGCTTAACGAACGGGCCAAACGATTTTATCTGAAGCTGGGGTTTATCCCGCTGGCAGGTGAAAACAGCAGTTCGCTGTTC
TTCCCCACGCAATCTATAGAACGACTGTTTGAGCAGGCGTGA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10247.75 Da        Isoelectric Point: 5.8836

>AT135871 WP_015570876.1 NZ_CP042551:1866382-1866651 [Enterobacter hormaechei]
MPALKKQRIDLRLSDEDKTMIEEAAAMTNQTITQFMVNSASERAAEVIEQHRRLVLNEASWIAVMDAIDNPPEPNERLKR
AAKRLRNME

Download         Length: 270 bp

>AT135871 NZ_CP042551:1866382-1866651 [Enterobacter hormaechei]
ATGCCAGCACTCAAAAAACAGCGTATCGATCTTCGCCTGTCGGACGAAGACAAAACAATGATTGAAGAAGCGGCAGCGAT
GACCAACCAAACGATCACCCAGTTTATGGTGAACAGTGCGTCAGAGCGTGCTGCCGAAGTCATCGAACAGCATCGGCGTC
TCGTATTAAACGAAGCCTCATGGATTGCCGTTATGGATGCCATCGACAATCCGCCAGAGCCAAATGAACGCCTGAAGCGA
GCGGCGAAACGTCTTCGAAACATGGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A222RDD1

Antitoxin

Source ID Structure
AlphaFold DB A0A331QJY7

References