Detailed information of TA system TA135866

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 294473..295069
• Replicon: chromosome
• Accession: NZ_CP042551
• Organism: Enterobacter hormaechei strain C45

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: FR772_RS01410
• Protein ID: WP_023295756.1
• Coordinates: 294767..295069 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: A0A927HL08
• Locus tag: FR772_RS01405
• Protein ID: WP_023295755.1
• Coordinates: 294473..294760 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FR772_RS01400	292845..294476	+	1632	WP_003860350.1	Na/Pi cotransporter family protein	-
FR772_RS01405	294473..294760	-	288	WP_023295755.1	putative addiction module antidote protein	Antitoxin
FR772_RS01410	294767..295069	-	303	WP_023295756.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FR772_RS01415	295267..296139	+	873	WP_032646867.1	23S rRNA pseudouridine(2604) synthase RluF	-
FR772_RS01420	296140..296412	-	273	WP_017382612.1	DUF3811 domain-containing protein	-
FR772_RS01425	296463..297407	-	945	WP_032646868.1	ketopantoate/pantoate/pantothenate transporter PanS	-
FR772_RS01430	297501..298850	-	1350	WP_006810501.1	lysine-sensitive aspartokinase 3	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-93)

Antitoxin

• Uncharacterized protein HI1420 (3-90)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11441.24 Da
• Isoelectric Point: 10.1042

>T135866 WP_023295756.1 NZ_CP042551:c295069-294767 [Enterobacter hormaechei]
MKEIVQTESFRRWEQNLKDRRAKTIIASRLFRLANGLAGDIRPVGEGISELRIHFGPGYRIYFKDQGNCIIVLLCGGDKS
SQARDILMAKMLSNVSQWQE

Nucleotide

• Length: 303 bp

>T135866 NZ_CP042551:c295069-294767 [Enterobacter hormaechei]
ATGAAGGAGATCGTTCAGACAGAATCCTTCCGACGCTGGGAGCAAAATTTAAAGGACAGGCGGGCAAAGACGATTATCGC
CTCCCGCCTCTTTAGGCTGGCAAATGGCTTAGCGGGCGACATTAGACCTGTGGGAGAAGGTATCAGTGAACTGAGGATTC
ACTTTGGCCCGGGCTACAGGATCTATTTTAAGGACCAGGGCAATTGCATCATCGTGCTGTTATGTGGTGGTGACAAAAGC
AGCCAGGCCAGAGACATACTTATGGCAAAAATGCTGAGCAATGTATCCCAATGGCAGGAGTGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10101.59 Da
• Isoelectric Point: 5.7807

>AT135866 WP_023295755.1 NZ_CP042551:c294760-294473 [Enterobacter hormaechei]
MHKLTPYDPANALLDDEEIAVFMADALETGDSAYIAKALGVIARAKGMSTISQQTGLSREQLYRSFSDKGNPTLKTTLAV
MKALGLGLTIKPSGD

Nucleotide

• Length: 288 bp

>AT135866 NZ_CP042551:c294760-294473 [Enterobacter hormaechei]
ATGCATAAATTAACACCCTACGATCCAGCCAACGCACTGCTGGATGACGAGGAAATCGCTGTGTTTATGGCTGATGCATT
AGAGACGGGTGACTCAGCGTACATTGCTAAAGCGCTGGGCGTCATCGCCCGAGCGAAAGGGATGTCGACCATTTCCCAGC
AAACGGGCCTGTCACGAGAACAACTGTATCGATCATTCAGTGATAAGGGGAACCCAACGCTCAAAACCACGCTGGCGGTC
ATGAAAGCATTGGGCCTTGGGTTAACAATCAAACCCTCTGGGGATTAA