Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 55022..55665 Replicon plasmid pC52_002
Accession NZ_CP042547
Organism Klebsiella michiganensis strain C52

Toxin (Protein)


Gene name vagD Uniprot ID A0A5P6KDG5
Locus tag FR848_RS29380 Protein ID WP_021312768.1
Coordinates 55249..55665 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID A0A5P6KGW8
Locus tag FR848_RS29375 Protein ID WP_021312767.1
Coordinates 55022..55252 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FR848_RS29345 50061..50990 + 930 WP_040118542.1 Rpn family recombination-promoting nuclease/putative transposase -
FR848_RS29350 51782..52477 + 696 WP_040118530.1 replication initiation protein -
FR848_RS29355 52522..53304 - 783 WP_040118529.1 site-specific integrase -
FR848_RS29360 53301..54044 - 744 WP_040118528.1 hypothetical protein -
FR848_RS29365 54089..54406 - 318 WP_047722684.1 hypothetical protein -
FR848_RS29375 55022..55252 + 231 WP_021312767.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
FR848_RS29380 55249..55665 + 417 WP_021312768.1 type II toxin-antitoxin system VapC family toxin Toxin
FR848_RS29390 55853..56557 - 705 WP_001067855.1 IS6-like element IS26 family transposase -
FR848_RS29400 56622..59396 + 2775 Protein_63 Tn3 family transposase -
FR848_RS29405 59604..60572 - 969 WP_077255425.1 IS5 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..173103 173103


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-50)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15175.60 Da        Isoelectric Point: 7.2452

>T135865 WP_021312768.1 NZ_CP042547:55249-55665 [Klebsiella michiganensis]
VNKIYMLDTNICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPDLVLEDWVR

Download         Length: 417 bp

>T135865 NZ_CP042547:55249-55665 [Klebsiella michiganensis]
GTGAACAAGATCTATATGCTCGACACCAACATCTGCTCGTTCATCATGCGCGAGCAGCCTGAAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGTGGCCACCGTATCGTGGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGTGCCACCGGCC
CGAAGGCCTCGCCGCGCCACGTCCAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGACCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGCCTCGCCGGAACGCCGATAGGCCCGAACGACACGGCGATCGC
CGGGCATGCCATTGCGGCCGGCGCCGTACTGGTGACAAATAATGTGCGGGAGTTTGAGCGGGTGCCGGATCTGGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8722.81 Da        Isoelectric Point: 4.4692

>AT135865 WP_021312767.1 NZ_CP042547:55022-55252 [Klebsiella michiganensis]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQLEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT135865 NZ_CP042547:55022-55252 [Klebsiella michiganensis]
ATGAGAACCGTATCTATTTTTAAAAACGGCAACAACCGCGCCATCCGCCTTCCCCGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGTCCCGTCCGGCCGACCTGGGGGTCGTTCGCGCAGCTCG
AAAAAGCCGATCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5P6KDG5

Antitoxin

Source ID Structure
AlphaFold DB A0A5P6KGW8

References