Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 56802..57538 Replicon plasmid pC52_001
Accession NZ_CP042546
Organism Klebsiella michiganensis strain C52

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag FR848_RS28285 Protein ID WP_003026803.1
Coordinates 57056..57538 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag FR848_RS28280 Protein ID WP_003026799.1
Coordinates 56802..57068 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FR848_RS28255 52996..54285 + 1290 WP_000922630.1 arsenite efflux transporter membrane subunit ArsB -
FR848_RS28260 54298..54723 + 426 WP_000065758.1 glutaredoxin-dependent arsenate reductase -
FR848_RS28265 54754..55020 - 267 WP_102047566.1 hypothetical protein -
FR848_RS28270 55094..55798 + 705 WP_001067855.1 IS6-like element IS26 family transposase -
FR848_RS28275 55744..56532 - 789 WP_077255422.1 hypothetical protein -
FR848_RS28280 56802..57068 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
FR848_RS28285 57056..57538 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
FR848_RS28290 57738..58655 + 918 Protein_62 ISNCY family transposase -
FR848_RS28295 58745..60244 + 1500 WP_004118622.1 IS21 family transposase -
FR848_RS28300 60241..60996 + 756 WP_038423655.1 IS21-like element ISEc10 family helper ATPase IstB -
FR848_RS28305 61063..61512 + 450 Protein_65 ISNCY family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..195216 195216
- inside IScluster/Tn - - 55094..63861 8767


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T135864 WP_003026803.1 NZ_CP042546:57056-57538 [Klebsiella michiganensis]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T135864 NZ_CP042546:57056-57538 [Klebsiella michiganensis]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT135864 WP_003026799.1 NZ_CP042546:56802-57068 [Klebsiella michiganensis]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT135864 NZ_CP042546:56802-57068 [Klebsiella michiganensis]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References