Detailed information of TA system TA135861

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 5501358..5501934
• Replicon: chromosome
• Accession: NZ_CP042545
• Organism: Klebsiella michiganensis strain C52

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: FR848_RS26135
• Protein ID: WP_038424021.1
• Coordinates: 5501647..5501934 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: A0A0H3H591
• Locus tag: FR848_RS26130
• Protein ID: WP_014227757.1
• Coordinates: 5501358..5501660 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FR848_RS26115	5498198..5498533	+	336	WP_014227760.1	endoribonuclease SymE	-
FR848_RS26120	5498986..5499897	+	912	WP_032719936.1	acetamidase/formamidase family protein	-
FR848_RS26125	5499894..5501237	+	1344	WP_032719935.1	APC family permease	-
FR848_RS26130	5501358..5501660	-	303	WP_014227757.1	BrnA antitoxin family protein	Antitoxin
FR848_RS26135	5501647..5501934	-	288	WP_038424021.1	BrnT family toxin	Toxin
FR848_RS26140	5502184..5502627	-	444	WP_014837312.1	FosA family fosfomycin resistance glutathione transferase	-
FR848_RS26145	5502621..5503529	-	909	WP_014837311.1	LysR family transcriptional regulator	-
FR848_RS26150	5503617..5504399	+	783	WP_014837310.1	NAD(P)H-dependent oxidoreductase	-
FR848_RS26155	5504547..5505131	+	585	WP_004098242.1	TetR/AcrR family transcriptional regulator	-
FR848_RS26160	5505277..5506077	+	801	WP_014837308.1	winged helix-turn-helix domain-containing protein	-
FR848_RS26165	5506074..5506592	+	519	WP_004098240.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (38-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11253.72 Da
• Isoelectric Point: 8.5899

>T135861 WP_038424021.1 NZ_CP042545:c5501934-5501647 [Klebsiella michiganensis]
MPMEFEWDANKARSNLRKHGIRFEEAVLVFDDPRHLSRQDRYENGEYRWQTLGLVHGIIVIMVAHSVRFESGTEVIRIIS
ARKADSKERNRYEHG

Nucleotide

• Length: 288 bp

>T135861 NZ_CP042545:c5501934-5501647 [Klebsiella michiganensis]
ATGCCAATGGAGTTTGAATGGGATGCGAACAAGGCAAGAAGTAATCTAAGAAAACATGGAATCCGGTTTGAGGAAGCGGT
GCTCGTCTTTGACGATCCCCGGCATCTTTCCCGACAGGATCGTTATGAGAATGGTGAATATCGCTGGCAAACTCTCGGCC
TCGTTCATGGGATCATTGTTATCATGGTTGCGCATTCGGTCCGTTTTGAAAGTGGAACAGAGGTCATTCGTATTATCAGC
GCCCGGAAGGCGGACAGCAAAGAGAGAAATCGTTATGAACATGGTTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11607.18 Da
• Isoelectric Point: 9.4429

>AT135861 WP_014227757.1 NZ_CP042545:c5501660-5501358 [Klebsiella michiganensis]
MNMVKHKRDNPDTLTAKRDDELKALANKTDDEIDYSDIPATDDAQWSDAVRGKFYRPLKTQASVRIDADIMEWLKRPGKG
YQTRLNAILREAMIRDLNKK

Nucleotide

• Length: 303 bp

>AT135861 NZ_CP042545:c5501660-5501358 [Klebsiella michiganensis]
ATGAACATGGTTAAACATAAACGTGACAATCCGGATACGCTTACGGCTAAGCGAGACGATGAGCTTAAAGCGCTGGCCAA
CAAAACGGATGATGAAATCGATTATAGCGATATTCCCGCCACAGACGACGCACAGTGGTCAGATGCCGTACGCGGTAAAT
TTTACCGACCGCTAAAAACTCAGGCTTCTGTCCGTATTGATGCCGATATTATGGAGTGGCTTAAACGTCCCGGGAAAGGT
TATCAAACGCGACTGAATGCGATCTTAAGAGAAGCGATGATTCGCGATCTTAACAAGAAATAG

Structures

Antitoxin

• AlphaFold DB: A0A0H3H591