Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4811938..4812557 Replicon chromosome
Accession NZ_CP042545
Organism Klebsiella michiganensis strain C52

Toxin (Protein)


Gene name Hha Uniprot ID H3N9D8
Locus tag FR848_RS22925 Protein ID WP_004099646.1
Coordinates 4812339..4812557 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID H3N7X7
Locus tag FR848_RS22920 Protein ID WP_004129911.1
Coordinates 4811938..4812312 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FR848_RS22910 4807095..4808288 + 1194 WP_004136054.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
FR848_RS22915 4808311..4811457 + 3147 WP_014228207.1 multidrug efflux RND transporter permease subunit -
FR848_RS22920 4811938..4812312 + 375 WP_004129911.1 Hha toxicity modulator TomB Antitoxin
FR848_RS22925 4812339..4812557 + 219 WP_004099646.1 hemolysin expression modulator Hha Toxin
FR848_RS22930 4812720..4813286 + 567 WP_038424164.1 maltose O-acetyltransferase -
FR848_RS22935 4813413..4813883 + 471 WP_014228205.1 YlaC family protein -
FR848_RS22940 4813858..4815312 - 1455 WP_038424163.1 PLP-dependent aminotransferase family protein -
FR848_RS22945 4815414..4816112 + 699 WP_014228203.1 GNAT family N-acetyltransferase -
FR848_RS22950 4816109..4816249 - 141 WP_003859006.1 type B 50S ribosomal protein L36 -
FR848_RS22955 4816249..4816512 - 264 WP_004848323.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8640.05 Da        Isoelectric Point: 8.9008

>T135859 WP_004099646.1 NZ_CP042545:4812339-4812557 [Klebsiella michiganensis]
MSDKTLTKIDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPPSVWKFIR

Download         Length: 219 bp

>T135859 NZ_CP042545:4812339-4812557 [Klebsiella michiganensis]
ATGTCTGATAAAACATTAACCAAAATAGACTATTTGATGCGTTTACGACGCTGTCAGACAATTGATACGCTGGAGCGCGT
AATTGAAAAAAATAAATATGAACTTTCCGATAATGAACTGGCGGTATTTTACTCTGCGGCCGATCATCGTCTTGCAGAGC
TGACGATGAATAAACTGTATGACAAGATCCCCCCTTCAGTCTGGAAATTTATCCGCTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14350.09 Da        Isoelectric Point: 4.8989

>AT135859 WP_004129911.1 NZ_CP042545:4811938-4812312 [Klebsiella michiganensis]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIAAFALNYKIKYAEDNKLITQIDEYL
DDTFVLFSNYGINSADLQKWRKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT135859 NZ_CP042545:4811938-4812312 [Klebsiella michiganensis]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCGCAGCTTAAATTTCTCTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTGGAAGAGAGCAACCACGGCTGGGTTAATGATCCAACCTCGGCTGTCAATCTTCAACTTAATGAATTGATCGAGC
ACATTGCCGCGTTTGCACTTAATTATAAAATTAAGTACGCAGAGGATAATAAGCTGATTACCCAGATTGACGAATATCTG
GATGACACTTTCGTCTTGTTCAGCAACTACGGCATTAACAGCGCAGATCTGCAGAAATGGCGTAAATCTGGCAATCGGTT
ATTCCGCTGCTTTGTCAACGCCAGTCGGGAAAACCCGGCCAGTCTTTCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3H713

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3HD25

References