Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 2432813..2433403 Replicon chromosome
Accession NZ_CP042545
Organism Klebsiella michiganensis strain C52

Toxin (Protein)


Gene name pemK Uniprot ID A0A0H3HDW1
Locus tag FR848_RS11740 Protein ID WP_014229979.1
Coordinates 2433071..2433403 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID J6I3K7
Locus tag FR848_RS11735 Protein ID WP_004852307.1
Coordinates 2432813..2433070 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FR848_RS11700 2428069..2428677 - 609 WP_014229984.1 glutathione S-transferase family protein -
FR848_RS11705 2428854..2429780 + 927 WP_014229983.1 LysR family transcriptional regulator -
FR848_RS11710 2429818..2430816 - 999 WP_014229982.1 aldo/keto reductase -
FR848_RS11715 2430917..2431831 + 915 WP_014229981.1 LysR family transcriptional regulator -
FR848_RS11720 2432022..2432102 - 81 Protein_2239 molybdopterin-binding protein -
FR848_RS11735 2432813..2433070 + 258 WP_004852307.1 antitoxin Antitoxin
FR848_RS11740 2433071..2433403 + 333 WP_014229979.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
FR848_RS11745 2433758..2434600 + 843 WP_038424696.1 hypothetical protein -
FR848_RS11750 2434776..2434994 + 219 WP_038424695.1 AlpA family transcriptional regulator -
FR848_RS11755 2435134..2435451 + 318 WP_038424694.1 hypothetical protein -
FR848_RS11760 2435517..2435753 + 237 WP_000999357.1 hypothetical protein -
FR848_RS11765 2435819..2436115 + 297 WP_000032534.1 hypothetical protein -
FR848_RS11770 2436178..2436414 + 237 WP_038424693.1 DNA-binding protein -
FR848_RS11775 2436463..2436930 - 468 WP_038424692.1 DUF4756 family protein -
FR848_RS11780 2437032..2437733 - 702 WP_038424691.1 MarR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-109)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11740.57 Da        Isoelectric Point: 10.1863

>T135850 WP_014229979.1 NZ_CP042545:2433071-2433403 [Klebsiella michiganensis]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPITSGGNFARTAGFTVSLEGAGTKTTGVIRCDQPRTI
DMAARNGKRLESIPDAVVNEVLARLDAILS

Download         Length: 333 bp

>T135850 NZ_CP042545:2433071-2433403 [Klebsiella michiganensis]
ATGGACAGAGGGGAAATCTGGCTCGTTTCACTGGATCCGATTGCTGGCCACGAGCAAAGCGGAAAACGTCCGGTGCTTAT
CGTATCGAAGGCATCGTTTAACAAGCTGACCCGGTTACCCGTTGTTGTTCCCATCACCAGCGGTGGAAATTTTGCTCGCA
CGGCAGGTTTCACCGTTTCACTGGAAGGGGCAGGGACAAAAACAACGGGCGTTATTCGCTGCGACCAGCCCAGAACCATT
GATATGGCTGCCCGGAACGGCAAGCGGCTGGAAAGCATACCAGACGCTGTGGTAAATGAAGTGCTTGCCAGGCTGGATGC
AATTTTAAGTTAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9422.88 Da        Isoelectric Point: 4.4607

>AT135850 WP_004852307.1 NZ_CP042545:2432813-2433070 [Klebsiella michiganensis]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTINNGCLIIEPQKRPHYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Download         Length: 258 bp

>AT135850 NZ_CP042545:2432813-2433070 [Klebsiella michiganensis]
ATGTATACCACTCGCCTGAAAAAGGTCGGCGGATCCATCATGTTGGCGGTTCCTCCCGCCGTGCTCAAAACGCTGGAGCT
GTCGACGGACAGCGAAGTCGGCATGACCATTAATAATGGCTGCCTGATTATTGAACCCCAGAAACGGCCTCATTATTCTC
TTGAGGAGCTGCTGGCGCAGTGCGATCCGCACGCCGAAATGAGCGAAGAGGATCGGGAATGGATTGATGCTCCTGCGGTG
GGCAAGGAGATCCTGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3HDW1

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3HDW8

References