Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 417964..418511 Replicon chromosome
Accession NZ_CP042545
Organism Klebsiella michiganensis strain C52

Toxin (Protein)


Gene name ccdB Uniprot ID A0A0H3H5T3
Locus tag FR848_RS01975 Protein ID WP_014227299.1
Coordinates 417964..418272 (-) Length 103 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A0H3H0P7
Locus tag FR848_RS01980 Protein ID WP_014227298.1
Coordinates 418275..418511 (-) Length 79 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FR848_RS01945 413611..413922 - 312 WP_038423882.1 PTS sugar transporter subunit IIB -
FR848_RS01950 414217..415158 + 942 WP_014227303.1 LacI family transcriptional regulator -
FR848_RS01955 415182..415475 - 294 WP_014227302.1 hypothetical protein -
FR848_RS01960 415685..416650 + 966 WP_038423881.1 Rpn family recombination-promoting nuclease/putative transposase -
FR848_RS01965 416650..416907 + 258 WP_032718834.1 hypothetical protein -
FR848_RS01970 416911..417813 - 903 WP_009654083.1 DMT family transporter -
FR848_RS01975 417964..418272 - 309 WP_014227299.1 CcdB family protein Toxin
FR848_RS01980 418275..418511 - 237 WP_014227298.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
FR848_RS01985 418617..420050 - 1434 WP_038423880.1 PTS N-acetylmuramic acid transporter subunit IIBC -
FR848_RS01990 420075..420977 - 903 WP_038423879.1 N-acetylmuramic acid 6-phosphate etherase -
FR848_RS01995 421138..422304 - 1167 WP_014839828.1 multidrug effflux MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-101)

Antitoxin

Post-segregation antitoxin CcdA

(8-78)


Sequences

Toxin        


Download         Length: 103 a.a.        Molecular weight: 11725.57 Da        Isoelectric Point: 7.2729

>T135848 WP_014227299.1 NZ_CP042545:c418272-417964 [Klebsiella michiganensis]
MQYYVYKNTGRIAAYPYLLDVQSDIIGKRNTRVVIPLFPLKNYKGPRADRLTPLVTVEGEEYVVMTHELASIPHRVLGEE
VCNLNHQREVVKASMDFLFDGI

Download         Length: 309 bp

>T135848 NZ_CP042545:c418272-417964 [Klebsiella michiganensis]
ATGCAGTATTACGTTTATAAGAATACAGGTCGAATTGCTGCGTATCCTTACCTCCTTGATGTACAGAGTGACATTATCGG
CAAGCGCAATACTCGGGTCGTGATTCCACTGTTTCCACTGAAAAACTATAAAGGTCCACGCGCCGATCGTTTAACACCGC
TTGTCACCGTAGAAGGGGAAGAATACGTCGTGATGACTCACGAACTCGCCAGTATCCCACATCGTGTTCTGGGTGAAGAA
GTTTGTAATCTGAATCACCAGCGCGAAGTCGTTAAAGCTTCAATGGATTTTCTTTTCGACGGGATCTAA

Antitoxin


Download         Length: 79 a.a.        Molecular weight: 8783.92 Da        Isoelectric Point: 5.7928

>AT135848 WP_014227298.1 NZ_CP042545:c418511-418275 [Klebsiella michiganensis]
MPAAKRNKQNVSVTLDPGLVKQAREVGINLSLTLTNALKSEIHSLEIARWQKENAQAIDYLNNLTEENGLLSDEYRTF

Download         Length: 237 bp

>AT135848 NZ_CP042545:c418511-418275 [Klebsiella michiganensis]
ATGCCAGCCGCAAAACGCAATAAGCAGAACGTCAGCGTGACACTGGACCCAGGCCTGGTCAAACAGGCTCGTGAGGTCGG
CATCAATCTGTCTCTAACGTTGACTAACGCTCTAAAAAGCGAAATCCACAGCCTCGAAATAGCCCGCTGGCAAAAAGAAA
ATGCCCAGGCAATCGACTACCTCAATAATTTAACTGAGGAAAATGGACTTCTCTCTGATGAATACAGGACGTTCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3H5T3

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3H0P7

References