Detailed information of TA system TA135845

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/ElaA-DUF1778
• Location: 72086..72825
• Replicon: plasmid pC4_001
• Accession: NZ_CP042541
• Organism: Enterobacter hormaechei strain C4

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A5E1AVR5
• Locus tag: FR758_RS23090
• Protein ID: WP_013087172.1
• Coordinates: 72340..72825 (+)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: A0A5E1AWX8
• Locus tag: FR758_RS23085
• Protein ID: WP_012540086.1
• Coordinates: 72086..72352 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FR758_RS23055	67947..69713	-	1767	Protein_71	Tn3 family transposase	-
FR758_RS23065	69765..70469	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
FR758_RS23070	70523..70738	-	216	Protein_73	RNA polymerase subunit sigma-70	-
FR758_RS23075	70747..71460	-	714	WP_013087170.1	arsenical resistance protein ArsH	-
FR758_RS23080	71462..71785	-	324	WP_013087171.1	metalloregulator ArsR/SmtB family transcription factor	-
FR758_RS23085	72086..72352	+	267	WP_012540086.1	DUF1778 domain-containing protein	Antitoxin
FR758_RS23090	72340..72825	+	486	WP_013087172.1	GNAT family N-acetyltransferase	Toxin
FR758_RS23095	73244..74584	+	1341	WP_000589001.1	ISNCY family transposase	-
FR758_RS23100	74840..75262	-	423	WP_032072095.1	nickel resistance OB fold protein NcrY	-
FR758_RS23105	75424..76554	-	1131	WP_004196366.1	Ni(II)/Co(II) efflux transporter permease subunit NcrC	-
FR758_RS23110	76567..76836	-	270	WP_004196355.1	nickel-sensing transcriptional repressor NcrB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	dfrA14 / catA2 / blaTEM-1B / aph(6)-Id / aph(3'')-Ib / sul2	-	1..155361	155361

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (27-152)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-87)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17810.62 Da
• Isoelectric Point: 9.8560

>T135845 WP_013087172.1 NZ_CP042541:72340-72825 [Enterobacter hormaechei]
VGRVTVPEPLSSFHQVAEFVCGETVLDDWLKQKGLKNQALGSARTFVVCKKDTKQVAGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDLSFRGKGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKNFYIHHGFKPSQTQQRTLFLKLP
Q

Nucleotide

• Length: 486 bp

>T135845 NZ_CP042541:72340-72825 [Enterobacter hormaechei]
GTGGGACGTGTAACAGTACCAGAACCTTTATCCAGCTTTCATCAGGTGGCGGAATTCGTTTGCGGTGAAACTGTTCTGGA
TGACTGGTTAAAGCAGAAAGGCCTCAAAAACCAGGCTCTCGGATCGGCCAGAACGTTTGTAGTGTGTAAAAAAGACACGA
AACAAGTAGCCGGTTTTTACTCTCTGGCCACCGGGAGCGTCAACCATACAGAAGCGACAGGCAATCTTCGGCGTAACATG
CCCGATCCCATCCCTGTTATTATACTTGCCCGTCTTGCTGTCGATCTCTCTTTCCGCGGAAAAGGGCTTGGCGCTGATTT
ACTTCATGATGCAGTGCTTCGTTGCTATCGGGTTGCCGAGAATATTGGTGTACGTGCAATCATGGTTCATGCACTCACCG
AAGAAGCTAAAAATTTCTACATTCACCATGGTTTCAAACCATCACAAACTCAGCAACGGACATTATTTCTTAAACTCCCT
CAATAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10076.47 Da
• Isoelectric Point: 4.3721

>AT135845 WP_012540086.1 NZ_CP042541:72086-72352 [Enterobacter hormaechei]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVADDPAIDKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT135845 NZ_CP042541:72086-72352 [Enterobacter hormaechei]
ATGAAATCAGATGTTCAACTTAATCTTAGAGCAAAGGAATCACAGCGGGCGCTCATTGATGCTGCTGCGGAAATTCTTCA
CAAATCACGAACAGACTTCATCCTGGAAATGGCCTGTCAGGCCGCTGAGAACGTGATCCTCGATCGCCGGGTCTTTAATT
TTAACGATGAACAATATGCCGAATTCATCGACATGCTTGATGCCCCTGTTGCAGATGATCCTGCCATAGATAAACTGCTG
GCAAGGAAACCTCAGTGGGACGTGTAA

Structures

Toxin

• AlphaFold DB: A0A5E1AVR5

Antitoxin

• AlphaFold DB: A0A5E1AWX8