Detailed information of TA system TA135843

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 4636379..4636995
• Replicon: chromosome
• Accession: NZ_CP042540
• Organism: Enterobacter hormaechei strain C4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: FR758_RS22245
• Protein ID: WP_015569913.1
• Coordinates: 4636379..4636750 (-)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A6G4MTK3
• Locus tag: FR758_RS22250
• Protein ID: WP_015569912.1
• Coordinates: 4636753..4636995 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FR758_RS22230	4633879..4634781	+	903	WP_014072386.1	formate dehydrogenase subunit beta	-
FR758_RS22235	4634778..4635413	+	636	WP_015569914.1	formate dehydrogenase cytochrome b556 subunit	-
FR758_RS22240	4635410..4636339	+	930	WP_003861956.1	formate dehydrogenase accessory protein FdhE	-
FR758_RS22245	4636379..4636750	-	372	WP_015569913.1	PIN domain-containing protein	Toxin
FR758_RS22250	4636753..4636995	-	243	WP_015569912.1	ribbon-helix-helix domain-containing protein	Antitoxin
FR758_RS22255	4637194..4638114	+	921	WP_015569911.1	alpha/beta hydrolase	-
FR758_RS22260	4638123..4639064	-	942	WP_015569910.1	fatty acid biosynthesis protein FabY	-
FR758_RS22265	4639109..4639546	-	438	WP_015569909.1	D-tyrosyl-tRNA(Tyr) deacylase	-
FR758_RS22270	4639543..4640424	-	882	WP_003861949.1	virulence factor BrkB family protein	-
FR758_RS22275	4640418..4641017	-	600	WP_017694048.1	glucose-1-phosphatase	-
FR758_RS22280	4641136..4641936	-	801	WP_003861944.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-115)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-49)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13767.90 Da
• Isoelectric Point: 6.4882

>T135843 WP_015569913.1 NZ_CP042540:c4636750-4636379 [Enterobacter hormaechei]
MEHMAVFDTNILIDLFNNRIEAADAIEHTASHRAISLITWMEVMVSARRHGHEAKTAAVMGAFEIIDVSRDIAERSVLLR
EKHGMKLPDAIILATAQSRKCPLISRNTKDFAGIDEVLTPYQV

Nucleotide

• Length: 372 bp

>T135843 NZ_CP055171:c53304-53017 [Shigella flexneri]
ATGACTTATACGGTAAAATTCAGGGATGATGCGCTCAAGGAATGGCTGAAACTGGATAAGACCATTCAACAACAGTTTGT
GAAAAAACTAAAAAAATGCAGCGAAAATCCTCATATACCGTCAGCAAAACTAAGAGGGCTAAAGGATTGCTACAAAATTA
AACTACGTGCATCGGGATTTCGTCTGGTTTATCAGGTGATTGACGATATGTTAATTATTGCGGTTGTTGCGGTAGGAAAA
CGAGAACGTAGTAACGTTTATAATCTCGCCAGTGAGAGAATGAGATAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9440.72 Da
• Isoelectric Point: 4.4076

>AT135843 WP_015569912.1 NZ_CP042540:c4636995-4636753 [Enterobacter hormaechei]
MSVLAELDMGRILIDLSDDVIQRLDNLKQLRNQPRAELLREAIELYLDQQSSSVIRNALGLWGDRKEDGLEYERKLREEW

Nucleotide

• Length: 243 bp

>AT135843 NZ_CP042540:c4636995-4636753 [Enterobacter hormaechei]
ATGAGCGTTTTGGCGGAACTTGATATGGGCAGAATACTTATCGATTTGTCTGATGACGTGATTCAACGTTTGGATAATCT
CAAGCAATTGCGAAATCAACCACGTGCTGAACTGCTTCGGGAAGCGATAGAGCTGTATCTCGACCAGCAGAGTTCTTCTG
TTATCCGAAATGCGCTTGGTCTGTGGGGAGACAGAAAAGAGGACGGGCTTGAGTATGAACGCAAGCTGCGTGAGGAGTGG
TAA

Structures

Antitoxin

• AlphaFold DB: A0A6G4MTK3