Detailed information of TA system TA135827

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prpT-prpA/ParE-CC2985
• Location: 2396..2945
• Replicon: plasmid pC9_001
• Accession: NZ_CP042531
• Organism: Klebsiella aerogenes strain C9

Toxin (Protein)

• Gene name: PrpT
• Uniprot ID: -
• Locus tag: FR830_RS24460
• Protein ID: WP_151585804.1
• Coordinates: 2396..2692 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: PrpA
• Uniprot ID: -
• Locus tag: FR830_RS24465
• Protein ID: WP_151585807.1
• Coordinates: 2679..2945 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FR830_RS24445	1..1011	+	1011	WP_151585798.1	RepB family plasmid replication initiator protein	-
FR830_RS24450	1292..2032	-	741	WP_151585801.1	site-specific integrase	-
FR830_RS24460	2396..2692	-	297	WP_151585804.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FR830_RS24465	2679..2945	-	267	WP_151585807.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
FR830_RS24470	3244..3401	+	158	Protein_4	replication initiation protein	-
FR830_RS24475	3512..3610	+	99	Protein_5	IS5/IS1182 family transposase	-
FR830_RS24480	3683..3994	+	312	WP_151585810.1	hypothetical protein	-
FR830_RS24485	4121..5686	-	1566	WP_192797129.1	PRD domain-containing protein	-
FR830_RS24490	5910..7466	+	1557	WP_151585817.1	PTS transporter subunit EIIC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..215014	215014

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-90)

Antitoxin

• Antitoxin ParD (4-74)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11546.05 Da
• Isoelectric Point: 6.2083

>T135827 WP_151585804.1 NZ_CP042531:c2692-2396 [Klebsiella aerogenes]
VKTVKITPKASQDLEDIWYYGQHHFGEQQADRYINQISDIFQVISAHNIGTPRPELGEYIFALPVERHMIYFLQTDTEII
IIRILSQHQDAGRHLNWQ

Nucleotide

• Length: 297 bp

>T135827 NZ_CP042531:c2692-2396 [Klebsiella aerogenes]
GTGAAAACCGTTAAAATCACGCCGAAAGCCAGTCAGGATCTGGAGGATATCTGGTATTACGGGCAGCACCATTTCGGTGA
ACAGCAGGCCGACAGATATATTAATCAGATATCCGACATTTTTCAGGTTATTAGTGCTCATAATATCGGCACTCCACGTC
CTGAACTGGGGGAATACATATTCGCACTACCCGTTGAACGACATATGATTTATTTTCTGCAAACTGATACTGAAATTATT
ATTATCCGTATATTAAGTCAGCATCAGGATGCCGGCCGTCATCTTAACTGGCAGTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10023.29 Da
• Isoelectric Point: 4.7605

>AT135827 WP_151585807.1 NZ_CP042531:c2945-2679 [Klebsiella aerogenes]
MARTMTVDLGDELREFIESLIESGDYRTQSEVIRESLRLLREKQAESRLQALRDLLAEGLSSGEPVEWEKDAFLKKVRAG
TRAAGENR

Nucleotide

• Length: 267 bp

>AT135827 NZ_CP042531:c2945-2679 [Klebsiella aerogenes]
ATGGCCAGAACAATGACAGTGGATCTCGGGGATGAACTCCGGGAGTTCATAGAATCCCTCATCGAATCAGGTGATTACCG
TACACAGAGTGAAGTAATCCGCGAGTCGCTTCGTCTCCTGCGCGAAAAACAGGCAGAGTCACGTCTCCAGGCCCTGCGGG
ATTTACTGGCAGAAGGCCTGAGCAGCGGCGAACCTGTGGAGTGGGAAAAGGATGCGTTCCTGAAAAAAGTCAGGGCTGGT
ACGAGGGCTGCCGGTGAAAACCGTTAA