Detailed information of TA system TA135824

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 2517822..2518561
• Replicon: chromosome
• Accession: NZ_CP042530
• Organism: Klebsiella aerogenes strain C9

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: FR830_RS12245
• Protein ID: WP_047048975.1
• Coordinates: 2518076..2518561 (+)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: A0A0H3FSM9
• Locus tag: FR830_RS12240
• Protein ID: WP_015705366.1
• Coordinates: 2517822..2518088 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FR830_RS12220	2514114..2514731	+	618	WP_045360840.1	response regulator transcription factor	-
FR830_RS12225	2514827..2515324	+	498	WP_045360838.1	heme-binding protein	-
FR830_RS12230	2515729..2516259	+	531	WP_058676118.1	N-acetyltransferase	-
FR830_RS12235	2516314..2517549	-	1236	WP_020078885.1	MFS transporter	-
FR830_RS12240	2517822..2518088	+	267	WP_015705366.1	DUF1778 domain-containing protein	Antitoxin
FR830_RS12245	2518076..2518561	+	486	WP_047048975.1	GNAT family N-acetyltransferase	Toxin
FR830_RS12250	2518633..2520249	+	1617	WP_047048973.1	FAD-NAD(P)-binding protein	-
FR830_RS12255	2520368..2521468	+	1101	WP_015366663.1	NADH:flavin oxidoreductase/NADH oxidase	-
FR830_RS12260	2521525..2521683	-	159	WP_002903230.1	YqaE/Pmp3 family membrane protein	-
FR830_RS12265	2521944..2523014	+	1071	WP_015705361.1	mannonate dehydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (27-151)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17587.26 Da
• Isoelectric Point: 8.8793

>T135824 WP_047048975.1 NZ_CP042530:2518076-2518561 [Klebsiella aerogenes]
MGKISAPAPLSSHHQIAEFCCGETVLDQWLKQRGLKNQAQGAARTFVVCKEESHQVVGFYSLATGSVNHTEATGGLRRNM
PDPIPVIILARLAIDCAFHGQGLGADLLHDALLRSYRVAENVGVRALMVHALTDSAKRFYLHHGFKASTTQERTLFLALP
K

Nucleotide

• Length: 486 bp

>T135824 NZ_CP042530:2518076-2518561 [Klebsiella aerogenes]
ATGGGAAAAATAAGCGCGCCTGCACCGCTATCTTCACACCATCAGATCGCGGAATTTTGTTGTGGAGAAACGGTTCTCGA
TCAATGGCTGAAACAGCGAGGATTGAAAAACCAGGCTCAGGGCGCCGCGAGAACTTTTGTGGTCTGCAAGGAAGAGAGCC
ATCAGGTGGTGGGGTTTTATTCGTTAGCAACCGGCAGCGTTAATCACACGGAAGCCACTGGCGGCCTGCGGCGCAATATG
CCCGATCCTATCCCGGTCATCATTCTCGCCCGCCTGGCGATCGACTGCGCGTTTCACGGACAGGGGCTGGGAGCTGATTT
ACTCCATGATGCGCTGTTACGCAGTTATCGCGTTGCCGAAAATGTTGGCGTACGTGCCCTGATGGTCCATGCCCTGACCG
ATAGCGCGAAACGATTCTATTTACACCACGGATTCAAAGCCTCAACCACCCAGGAACGCACTCTTTTCCTCGCGTTGCCT
AAATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10085.56 Da
• Isoelectric Point: 4.6558

>AT135824 WP_015705366.1 NZ_CP042530:2517822-2518088 [Klebsiella aerogenes]
MKSDIQLNIRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNLSDEQYAEFIDMLDAPVEANPALDKLL
ARKPQWEK

Nucleotide

• Length: 267 bp

>AT135824 NZ_CP055170:c834468-834208 [Shigella flexneri]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Structures

Antitoxin

• AlphaFold DB: A0A0H3FSM9