Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 272704..273335 Replicon chromosome
Accession NZ_CP042530
Organism Klebsiella aerogenes strain C9

Toxin (Protein)


Gene name hicA Uniprot ID A0A0H3FL31
Locus tag FR830_RS01235 Protein ID WP_015703741.1
Coordinates 272704..272979 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A0H3FNK6
Locus tag FR830_RS01240 Protein ID WP_015703740.1
Coordinates 272976..273335 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FR830_RS01215 267932..268267 + 336 WP_015703743.1 universal stress protein UspB -
FR830_RS01220 268333..269829 - 1497 WP_015369272.1 inorganic phosphate transporter PitA -
FR830_RS01225 270060..271253 + 1194 WP_072383310.1 NAD(P)/FAD-dependent oxidoreductase -
FR830_RS01230 271293..272306 - 1014 WP_059444462.1 magnesium transporter -
FR830_RS01235 272704..272979 + 276 WP_015703741.1 type II toxin-antitoxin system HicA family toxin Toxin
FR830_RS01240 272976..273335 + 360 WP_015703740.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
FR830_RS01245 273363..273764 - 402 WP_020077744.1 nickel-responsive transcriptional regulator NikR -
FR830_RS01250 273752..274543 - 792 WP_059444461.1 nickel import ATP-binding protein NikE -
FR830_RS01255 274540..275304 - 765 WP_059444460.1 nickel import ATP-binding protein NikD -
FR830_RS01260 275304..276137 - 834 WP_015369278.1 nickel ABC transporter permease subunit NikC -
FR830_RS01265 276134..277078 - 945 WP_015703735.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10258.89 Da        Isoelectric Point: 10.4888

>T135813 WP_015703741.1 NZ_CP042530:272704-272979 [Klebsiella aerogenes]
MEQQVLSLRKKQRNTLEQLFKTPVPQGIKWADIESLIKALGGEIKEGRGSRCKFLLNHSIASFHRPHPSPDTDKGAVESV
RDWLITIGVKP

Download         Length: 276 bp

>T135813 NZ_CP042530:272704-272979 [Klebsiella aerogenes]
ATGGAGCAGCAGGTTTTATCTTTACGTAAAAAACAGCGCAACACGCTGGAGCAGCTGTTTAAAACCCCCGTGCCGCAGGG
TATTAAATGGGCCGACATTGAATCGCTAATTAAAGCGTTAGGTGGGGAGATTAAAGAAGGACGCGGCTCGCGCTGTAAAT
TTTTGTTGAACCATAGCATTGCCAGCTTTCACAGGCCTCACCCTTCGCCGGATACTGACAAAGGCGCCGTGGAGAGCGTG
CGCGATTGGTTAATCACTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13305.02 Da        Isoelectric Point: 4.3472

>AT135813 WP_015703740.1 NZ_CP042530:272976-273335 [Klebsiella aerogenes]
MIKPKTPNSMEIAGQPAVINYVPELNAFRGKFLGLSGYCDFVSDSIQGLQQEGEISLQEYLADCREAGIEPYAQPEKLKT
FTLRYPESFGERLSSAAAEEQVSVNTWILETLNERLKQA

Download         Length: 360 bp

>AT135813 NZ_CP042530:272976-273335 [Klebsiella aerogenes]
ATGATCAAACCTAAAACGCCAAATTCAATGGAGATTGCCGGGCAGCCTGCTGTTATTAACTACGTGCCTGAACTCAATGC
ATTCCGCGGTAAGTTCCTGGGATTATCAGGATATTGCGATTTTGTTTCCGATAGCATTCAAGGGTTACAACAGGAGGGCG
AGATATCATTACAGGAATATCTTGCTGATTGTCGTGAAGCGGGCATCGAGCCTTATGCTCAGCCGGAAAAGCTGAAAACC
TTCACATTGCGTTATCCTGAATCATTTGGTGAGCGTCTTAGCTCCGCCGCTGCAGAGGAGCAGGTTTCCGTAAATACCTG
GATCCTTGAGACGCTCAATGAACGTCTCAAACAGGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3FL31

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3FNK6

References