Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 59459..60102 Replicon plasmid pE33_002
Accession NZ_CP042519
Organism Citrobacter freundii strain E33

Toxin (Protein)


Gene name vagD Uniprot ID A0A6C0NE46
Locus tag FR815_RS26265 Protein ID WP_008322233.1
Coordinates 59686..60102 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag FR815_RS26260 Protein ID WP_001261276.1
Coordinates 59459..59689 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FR815_RS26235 56635..56991 - 357 WP_000215515.1 cupin domain-containing protein -
FR815_RS26245 57394..58098 - 705 WP_001067848.1 IS6-like element IS26 family transposase -
FR815_RS26250 58184..59206 + 1023 WP_008322227.1 helicase UvrD -
FR815_RS26260 59459..59689 + 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
FR815_RS26265 59686..60102 + 417 WP_008322233.1 type II toxin-antitoxin system VapC family toxin Toxin
FR815_RS26270 60296..60465 + 170 Protein_58 DUF596 domain-containing protein -
FR815_RS26275 60648..61568 - 921 WP_008322235.1 carbohydrate kinase -
FR815_RS26280 61565..62935 - 1371 WP_008322237.1 hypothetical protein -
FR815_RS26285 63013..64032 - 1020 WP_004113181.1 ribose ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaIMP-4 / catB3 / blaOXA-10 / ant(3'')-Ia / blaVEB-3 / aac(6')-Ib-cr / blaOXA-1 / ARR-3 / qacE / sul1 / mph(A) / aac(3)-IId - 1..176400 176400


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15054.58 Da        Isoelectric Point: 9.2957

>T135791 WP_008322233.1 NZ_CP042519:59686-60102 [Citrobacter freundii]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAASAVLVTNNVREFARVPGLMLEDWVK

Download         Length: 417 bp

>T135791 NZ_CP042519:59686-60102 [Citrobacter freundii]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTACGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCTGTCCTGCCCTGGGACCGCGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCGGCCAGTGCCGTCCTGGTGACGAACAATGTGAGAGAGTTTGCGCGGGTCCCGGGCCTGATGCTGG
AAGACTGGGTAAAATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT135791 WP_001261276.1 NZ_CP042519:59459-59689 [Citrobacter freundii]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT135791 NZ_CP042519:59459-59689 [Citrobacter freundii]
ATGCGCACAGTTTCTATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCATTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6C0NE46

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References