Detailed information of TA system TA135790

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 219136..220704
• Replicon: plasmid pE33_001
• Accession: NZ_CP042518
• Organism: Citrobacter freundii strain E33

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: FR815_RS25810
• Protein ID: WP_151532702.1
• Coordinates: 219397..220704 (+)
• Length: 436 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: FR815_RS25805
• Protein ID: WP_012766359.1
• Coordinates: 219136..219387 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FR815_RS25775	215306..215758	+	453	WP_057521240.1	hypothetical protein	-
FR815_RS25780	215774..216376	-	603	WP_057521239.1	hypothetical protein	-
FR815_RS25785	216640..216908	+	269	Protein_238	hypothetical protein	-
FR815_RS25795	217218..217748	+	531	WP_057521237.1	hypothetical protein	-
FR815_RS25800	217751..218755	+	1005	WP_012766358.1	tyrosine-type recombinase/integrase	-
FR815_RS25805	219136..219387	+	252	WP_012766359.1	helix-turn-helix domain-containing protein	Antitoxin
FR815_RS25810	219397..220704	+	1308	WP_151532702.1	type II toxin-antitoxin system HipA family toxin	Toxin
FR815_RS25815	220701..221195	+	495	WP_057521236.1	hypothetical protein	-
FR815_RS25820	221370..222308	-	939	WP_057521235.1	hypothetical protein	-
FR815_RS25825	222507..224147	+	1641	WP_057521234.1	PAAR domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	formA	-	1..243455	243455

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (10-64)

Sequences

Toxin

Protein

• Length: 436 a.a.
• Molecular weight: 47840.99 Da
• Isoelectric Point: 6.1142

>T135790 WP_151532702.1 NZ_CP042518:219397-220704 [Citrobacter freundii]
MQTLTVAMNGEAVGTLYRDNKGAMSFQYAPEWISEPGSRAISMSLPLGHGRITGSEVFNFFSNLLPDSEAIIARMQARFR
VETTHPFDLLASVGRDCVGAIQLYSPDADIPTVMETLAEPLDEAQIEQLLEGYQEAPLGMAEDADFRISIAGAQEKTALL
WYQDRWQRPLGSTPTSHIFKLPIGKIVQNNIDLSESCENEWLCLRIAKAFGFDVANADLATFGKKKVLVVQRFDRRWSKS
GEWLLRLPQEDFCQALGIAPALKYESHGGPGIADAMKLLLGSRFAAKDREQFFKSQILFWMLAAIDGHGKNFSIFIEPAS
SFRMTPLYDVMSAFPIFKSGGIAAQKAKMGMALQGKNRQYSFAMIQPRHFISTAAHVGFPPDTAAQLMLDMATKTEEVVA
TVAAELPAGFPEHISSAIFEGLSSQAAKILKGVAK

Nucleotide

• Length: 1308 bp

>T135790 NZ_CP055162:3975221-3975439 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9380.82 Da
• Isoelectric Point: 9.8930

>AT135790 WP_012766359.1 NZ_CP042518:219136-219387 [Citrobacter freundii]
MKVTSPSLLAAAVRDQRKLSKLTQSEAAKQVGIKQTTVSDFELRPESTKLETLFKLLAALDLELHVVKRGSTLDDNKVWD
REW

Nucleotide

• Length: 252 bp

>AT135790 NZ_CP042518:219136-219387 [Citrobacter freundii]
ATGAAAGTGACATCTCCATCCTTACTTGCGGCTGCGGTGCGCGACCAACGCAAGCTCAGCAAACTAACGCAGTCAGAGGC
GGCGAAACAGGTCGGAATCAAGCAGACGACCGTCTCTGATTTTGAACTACGGCCAGAATCTACCAAGCTGGAGACGCTAT
TCAAGCTCCTTGCTGCACTGGATCTGGAGCTTCATGTCGTGAAGCGGGGTTCAACATTGGACGATAATAAAGTCTGGGAT
AGGGAGTGGTAA