Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 4242089..4242720 Replicon chromosome
Accession NZ_CP042517
Organism Citrobacter freundii strain E33

Toxin (Protein)


Gene name hicA Uniprot ID A0A0J1MPQ6
Locus tag FR815_RS20840 Protein ID WP_003837806.1
Coordinates 4242445..4242720 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A0J1MMS0
Locus tag FR815_RS20835 Protein ID WP_003837808.1
Coordinates 4242089..4242448 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FR815_RS20805 4237185..4237850 + 666 WP_119174639.1 7-cyano-7-deazaguanine/7-aminomethyl-7- deazaguanine transporter -
FR815_RS20810 4237923..4238480 + 558 WP_003837816.1 DcrB family lipoprotein -
FR815_RS20815 4238484..4239701 - 1218 WP_016151126.1 MFS transporter -
FR815_RS20820 4239835..4240884 + 1050 WP_003023393.1 AI-2E family transporter -
FR815_RS20825 4240947..4241525 + 579 WP_003837810.1 4'-phosphopantetheinyl transferase AcpT -
FR815_RS20830 4241575..4241976 + 402 WP_003023387.1 nickel-responsive transcriptional regulator NikR -
FR815_RS20835 4242089..4242448 - 360 WP_003837808.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
FR815_RS20840 4242445..4242720 - 276 WP_003837806.1 type II toxin-antitoxin system HicA family toxin Toxin
FR815_RS20845 4242798..4243922 - 1125 WP_003837801.1 ABC transporter permease -
FR815_RS20850 4243922..4246669 - 2748 WP_032937231.1 ribosome-associated ATPase/putative transporter RbbA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10210.88 Da        Isoelectric Point: 10.7228

>T135787 WP_003837806.1 NZ_CP042517:c4242720-4242445 [Citrobacter freundii]
MKEKVLSLRKKQKNTLDQLFKAPTPQGIKWSEIESLIKALGGEIKEGRGSRCKFLLNKSIASFHRPHPSPDTDKGAVENV
RDWLTSIGVKP

Download         Length: 276 bp

>T135787 NZ_CP042517:c4242720-4242445 [Citrobacter freundii]
ATGAAGGAAAAAGTGTTGTCTTTACGCAAAAAGCAAAAGAACACGCTGGATCAGCTTTTTAAAGCACCGACGCCGCAGGG
AATCAAGTGGTCGGAAATTGAGTCACTGATCAAGGCGTTAGGTGGCGAAATCAAAGAAGGACGAGGCTCGCGATGTAAAT
TTCTTTTAAATAAGAGCATTGCGAGTTTTCATAGACCACATCCTTCTCCGGATACAGATAAAGGCGCGGTCGAAAACGTA
CGTGACTGGTTAACAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13198.98 Da        Isoelectric Point: 4.5462

>AT135787 WP_003837808.1 NZ_CP042517:c4242448-4242089 [Citrobacter freundii]
MIKPKTPNSMEIAGQPAVINYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGEISLREYLDDCSAAGIEPYANPEKLKT
FTLRYPESFGERLSFAAAEEQVSVNTWIIETLSKSLKQA

Download         Length: 360 bp

>AT135787 NZ_CP042517:c4242448-4242089 [Citrobacter freundii]
ATGATCAAACCCAAAACGCCAAACTCAATGGAAATCGCCGGTCAACCTGCGGTGATTAATTATGTCCCTGAGCTTAATGC
GTTTCGCGGTAAGTTCCTGGGATTGTCAGGCTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAGGGGG
AGATCTCCTTACGAGAGTATCTTGATGATTGCTCTGCCGCAGGGATAGAACCTTACGCAAACCCGGAAAAGCTGAAGACG
TTTACTCTGCGCTATCCAGAGTCTTTCGGAGAACGTTTAAGTTTTGCGGCAGCGGAAGAACAGGTATCAGTCAATACATG
GATCATCGAAACGCTCAGCAAAAGTCTCAAACAGGCATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J1MPQ6

Antitoxin

Source ID Structure
AlphaFold DB A0A0J1MMS0

References