Detailed information of TA system TA135785

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3737111..3737914
• Replicon: chromosome
• Accession: NZ_CP042517
• Organism: Citrobacter freundii strain E33

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A6N6JYU3
• Locus tag: FR815_RS18285
• Protein ID: WP_024156451.1
• Coordinates: 3737534..3737914 (+)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A5A9BSL8
• Locus tag: FR815_RS18280
• Protein ID: WP_038807908.1
• Coordinates: 3737111..3737476 (+)
• Length: 122 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FR815_RS18235	3732177..3733064	+	888	WP_048242072.1	50S ribosome-binding GTPase	-
FR815_RS18240	3733270..3733671	+	402	WP_048242038.1	hypothetical protein	-
FR815_RS18245	3733768..3734235	+	468	WP_048242036.1	hypothetical protein	-
FR815_RS18250	3734426..3734686	+	261	WP_048242035.1	DUF905 family protein	-
FR815_RS18255	3734802..3735620	+	819	WP_048242033.1	DUF945 domain-containing protein	-
FR815_RS18265	3735889..3736362	+	474	WP_024156447.1	antirestriction protein	-
FR815_RS18270	3736375..3736854	+	480	WP_024156448.1	DNA repair protein RadC	-
FR815_RS18275	3736868..3737089	+	222	WP_024156449.1	DUF987 domain-containing protein	-
FR815_RS18280	3737111..3737476	+	366	WP_038807908.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
FR815_RS18285	3737534..3737914	+	381	WP_024156451.1	TA system toxin CbtA family protein	Toxin
FR815_RS18290	3737911..3738402	+	492	WP_024156452.1	hypothetical protein	-
FR815_RS18300	3738711..3739558	+	848	Protein_3505	DUF4942 domain-containing protein	-
FR815_RS18305	3740019..3741878	-	1860	WP_003838200.1	bifunctional glutathionylspermidine amidase/synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-110)

Antitoxin

• Antitoxin CbeA (15-114)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 14263.44 Da
• Isoelectric Point: 10.0919

>T135785 WP_024156451.1 NZ_CP042517:3737534-3737914 [Citrobacter freundii]
MQTLSAIPKRKAPSRPTPVEIWQQLLTYLLKRHYGLSLSDTQFSDEEIITQYIDAGISLSDALNFLVEKSELVRIDRPGF
SIKHQSPFIGVIDILRARRATGLMQRNGYKRITLLIAGNAAQEQHS

Nucleotide

• Length: 381 bp

>T135785 NZ_CP042517:3737534-3737914 [Citrobacter freundii]
ATGCAAACCTTATCCGCCATCCCGAAGCGGAAGGCACCGTCACGCCCGACGCCCGTTGAAATCTGGCAGCAACTTCTTAC
GTATCTGTTAAAAAGACATTACGGCCTTTCGCTTAGCGACACGCAATTTTCCGATGAGGAGATTATTACGCAATATATTG
ACGCCGGTATCTCACTGTCAGATGCACTCAATTTTCTCGTTGAAAAATCTGAACTGGTGCGGATTGATCGTCCCGGTTTC
TCCATTAAGCATCAGTCCCCTTTTATCGGGGTGATTGATATTCTCCGGGCCAGACGGGCAACGGGTTTGATGCAACGTAA
CGGGTATAAAAGGATCACTCTTCTCATCGCTGGAAATGCAGCTCAGGAGCAACATTCATGA

Antitoxin

Protein

• Length: 122 a.a.
• Molecular weight: 13344.20 Da
• Isoelectric Point: 5.8705

>AT135785 WP_038807908.1 NZ_CP042517:3737111-3737476 [Citrobacter freundii]
MSNPIPPVNHDVSEPWWGLKPGITPCFGARLVQEGNRLHYLADRASIAGVFSDADLRHLDQAFPVLLKQLELMLVSGELN
PCHQHCVTLYAKGLTCEADSLGSHGYIYTAIYPTPDDSITR

Nucleotide

• Length: 366 bp

>AT135785 NZ_CP042517:3737111-3737476 [Citrobacter freundii]
TTGTCGAATCCAATACCTCCAGTAAACCATGATGTCTCTGAGCCCTGGTGGGGGCTGAAACCGGGCATCACTCCGTGTTT
TGGCGCACGACTGGTACAGGAAGGCAACCGCCTGCATTATCTGGCTGATCGGGCCAGTATCGCCGGTGTGTTCAGCGATG
CGGATTTACGCCATCTCGATCAAGCATTTCCGGTACTGCTCAAACAGCTGGAATTAATGCTGGTTTCCGGTGAGCTCAAT
CCCTGCCATCAGCACTGTGTCACGTTATATGCAAAAGGGCTGACCTGTGAAGCAGATTCCCTCGGATCACATGGCTACAT
TTATACAGCAATTTATCCGACACCAGACGACAGCATAACCCGGTAA

Structures

Toxin

• AlphaFold DB: A0A6N6JYU3

Antitoxin

• AlphaFold DB: A0A5A9BSL8