Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 3566096..3566612 Replicon chromosome
Accession NZ_CP042517
Organism Citrobacter freundii strain E33

Toxin (Protein)


Gene name relE Uniprot ID A0A133L8K5
Locus tag FR815_RS17395 Protein ID WP_003844927.1
Coordinates 3566096..3566380 (-) Length 95 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A0J1MV10
Locus tag FR815_RS17400 Protein ID WP_003839576.1
Coordinates 3566370..3566612 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FR815_RS17380 3561322..3562974 + 1653 WP_119173899.1 alpha,alpha-phosphotrehalase -
FR815_RS17385 3563383..3565521 + 2139 WP_003844922.1 anaerobic ribonucleoside-triphosphate reductase -
FR815_RS17390 3565628..3566092 + 465 WP_032937736.1 anaerobic ribonucleoside-triphosphate reductase-activating protein -
FR815_RS17395 3566096..3566380 - 285 WP_003844927.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
FR815_RS17400 3566370..3566612 - 243 WP_003839576.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
FR815_RS17405 3566690..3568603 - 1914 WP_003844930.1 BglG family transcription antiterminator -
FR815_RS17410 3568625..3569365 - 741 WP_048216922.1 KDGP aldolase family protein -
FR815_RS17415 3569362..3570480 - 1119 WP_054527995.1 DgaE family pyridoxal phosphate-dependent ammonia lyase -
FR815_RS17420 3570464..3571597 - 1134 WP_060855415.1 amidohydrolase/deacetylase family metallohydrolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-82)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-69)


Sequences

Toxin        


Download         Length: 95 a.a.        Molecular weight: 10892.76 Da        Isoelectric Point: 10.0482

>T135784 WP_003844927.1 NZ_CP042517:c3566380-3566096 [Citrobacter freundii]
MTYELEFDPRALKEWHKLGDTVKAQFKKKLADVLLNPRIDSARLNGLPDCYKIKLKSSGYRLLYQVRDEVVIVFVVAVGK
REHSAVYLDANKRL

Download         Length: 285 bp

>T135784 NZ_CP042517:c3566380-3566096 [Citrobacter freundii]
ATGACTTATGAACTGGAATTCGACCCGAGAGCCTTAAAAGAGTGGCATAAACTGGGTGATACGGTGAAGGCTCAGTTTAA
GAAGAAGTTGGCCGACGTGTTGTTGAACCCCAGAATTGACTCTGCCCGTTTAAACGGTCTTCCAGATTGCTATAAAATTA
AGCTTAAATCGTCCGGCTATCGTCTGCTGTACCAGGTTCGGGATGAGGTTGTGATCGTGTTTGTTGTCGCGGTCGGCAAG
CGAGAACATTCGGCCGTTTATCTCGATGCAAACAAACGGCTTTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 8961.34 Da        Isoelectric Point: 5.0651

>AT135784 WP_003839576.1 NZ_CP042517:c3566612-3566370 [Citrobacter freundii]
MATLNVRLDDKLKNEAYAVLEKLNITPTEAVRLLFQYVAETGRMPVKTVTVSDSEDTLIQTVRERLASPQKGIKVSLDDL

Download         Length: 243 bp

>AT135784 NZ_CP042517:c3566612-3566370 [Citrobacter freundii]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCTGTGCGATTACTGTTTCAGTACGTTGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACCGTTAGCGACA
GCGAAGATACACTGATTCAGACAGTCCGGGAGAGGCTGGCCAGCCCGCAAAAAGGAATCAAGGTTAGTCTGGATGACTTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A133L8K5

Antitoxin

Source ID Structure
AlphaFold DB A0A0J1MV10

References