Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2905960..2906580 Replicon chromosome
Accession NZ_CP042517
Organism Citrobacter freundii strain E33

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag FR815_RS14300 Protein ID WP_002892050.1
Coordinates 2906362..2906580 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID R8WZW8
Locus tag FR815_RS14295 Protein ID WP_003021733.1
Coordinates 2905960..2906334 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FR815_RS14285 2901106..2902299 + 1194 WP_003835922.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
FR815_RS14290 2902322..2905471 + 3150 WP_003021736.1 multidrug efflux RND transporter permease subunit -
FR815_RS14295 2905960..2906334 + 375 WP_003021733.1 Hha toxicity modulator TomB Antitoxin
FR815_RS14300 2906362..2906580 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
FR815_RS14305 2906887..2907438 + 552 WP_003835924.1 maltose O-acetyltransferase -
FR815_RS14310 2907555..2908025 + 471 WP_087879033.1 YlaC family protein -
FR815_RS14315 2908104..2908244 - 141 WP_005125190.1 type B 50S ribosomal protein L36 -
FR815_RS14320 2908246..2908506 - 261 WP_003021719.1 type B 50S ribosomal protein L31 -
FR815_RS14325 2908695..2910248 + 1554 WP_003835927.1 EAL domain-containing protein -
FR815_RS14330 2910300..2910653 - 354 WP_003831033.1 DUF1428 family protein -
FR815_RS14335 2910718..2911347 - 630 WP_003835929.1 membrane protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T135783 WP_002892050.1 NZ_CP042517:2906362-2906580 [Citrobacter freundii]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T135783 NZ_CP042517:2906362-2906580 [Citrobacter freundii]
ATGTCCGATAAACCATTAACTAAAACAGATTATTTGATGCGCCTGCGACGTTGCCAGACAATTGATACGCTAGAGCGCGT
TATTGAAAAAAATAAATATGAATTGTCCGACAACGAACTGGCTGTATTTTACTCAGCAGCAGATCATCGTCTTGCTGAAT
TGACCATGAATAAGCTTTACGACAAGATTCCAACTTCTGTTTGGAAATTCATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14427.23 Da        Isoelectric Point: 5.5653

>AT135783 WP_003021733.1 NZ_CP042517:2905960-2906334 [Citrobacter freundii]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEQSNHGWVNDPTSATSLQLNELIEHIATFALNYKIKYNEDNKLIAQIDEYL
DDTFMLFSSYGINTQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Download         Length: 375 bp

>AT135783 NZ_CP042517:2905960-2906334 [Citrobacter freundii]
ATGGATGAATACTCACCCAAAAGACATGATATCGCGCAGCTTAAATTTCTCTGCGAAACTCTGTATCATGACTGTCTTGC
AAACCTTGAACAAAGTAACCATGGCTGGGTTAATGACCCTACCTCTGCAACCAGCTTGCAGCTCAATGAACTGATAGAGC
ATATTGCAACCTTCGCACTTAATTATAAAATTAAGTACAATGAGGACAATAAGCTGATCGCGCAGATAGATGAATACCTG
GATGACACTTTTATGTTGTTCAGCAGTTATGGTATTAATACGCAGGATCTGCAGAAATGGCGGAAGTCAGGTAATCGACT
GTTTCGCTGTTTTGTCAATGCCACCAGGGCTAATCCTGTTAGTTTGTCTTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB R8WZW8

References