Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 2160913..2161503 Replicon chromosome
Accession NZ_CP042517
Organism Citrobacter freundii strain E33

Toxin (Protein)


Gene name pemK Uniprot ID A0A0J1NBD3
Locus tag FR815_RS10660 Protein ID WP_003836692.1
Coordinates 2160913..2161245 (-) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A0J1NBD4
Locus tag FR815_RS10665 Protein ID WP_003836694.1
Coordinates 2161246..2161503 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FR815_RS10635 2156666..2158153 + 1488 WP_003846749.1 EmmdR/YeeO family multidrug/toxin efflux MATE transporter -
FR815_RS10645 2158469..2159419 - 951 WP_119174146.1 HTH-type transcriptional regulator Cbl -
FR815_RS10650 2159521..2160438 - 918 WP_044700649.1 nitrogen assimilation transcriptional regulator NAC -
FR815_RS10660 2160913..2161245 - 333 WP_003836692.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
FR815_RS10665 2161246..2161503 - 258 WP_003836694.1 hypothetical protein Antitoxin
FR815_RS10675 2162117..2166016 + 3900 WP_060855256.1 hypothetical protein -
FR815_RS10680 2166021..2166479 + 459 Protein_2049 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 2159521..2181827 22306


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-109)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11703.48 Da        Isoelectric Point: 8.5572

>T135782 WP_003836692.1 NZ_CP042517:c2161245-2160913 [Citrobacter freundii]
MDRGEIWLVSLDPTAGHEQSGKRPVLIVSPASFNKFTRLPVVVPVTSGGNFARAAGFTVSLDGAGTKTTGVIRCDQPRTI
DMGARNGKCLERIPEAVVNEVLARLEAILS

Download         Length: 333 bp

>T135782 NZ_CP042517:c2161245-2160913 [Citrobacter freundii]
ATGGACAGAGGGGAAATCTGGCTCGTCTCACTGGATCCTACAGCCGGACATGAGCAAAGCGGAAAACGTCCGGTGCTTAT
CGTTTCTCCAGCATCATTTAATAAGTTCACCCGGCTACCTGTTGTCGTTCCCGTCACTAGCGGCGGAAACTTTGCCCGCG
CAGCCGGTTTTACCGTCTCCCTGGACGGTGCGGGAACAAAAACAACGGGTGTTATTCGCTGCGACCAACCCAGAACGATT
GATATGGGGGCTCGGAACGGTAAGTGTCTGGAACGTATACCCGAAGCTGTTGTAAATGAAGTGCTGGCCCGACTGGAAGC
CATTCTAAGCTGA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9428.89 Da        Isoelectric Point: 4.3440

>AT135782 WP_003836694.1 NZ_CP042517:c2161503-2161246 [Citrobacter freundii]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTIDNGCLIIEPQKRPRYSLEELLAQCDPHAEMSDEDREWIDAPAV
GKEIL

Download         Length: 258 bp

>AT135782 NZ_CP042517:c2161503-2161246 [Citrobacter freundii]
ATGTATACCACTCGCCTGAAAAAGGTCGGCGGATCCATCATGCTGGCGGTTCCTCCTGCCGTGCTGAAAACGCTGGAGCT
GTCGACGGACAGCGAAGTGGGTATGACCATTGATAATGGCTGCCTGATTATTGAACCCCAGAAACGACCTCGTTATTCGC
TTGAGGAACTGCTGGCGCAGTGCGATCCGCACGCCGAAATGAGCGATGAGGATAGGGAATGGATTGATGCGCCTGCAGTG
GGTAAGGAGATCCTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J1NBD3

Antitoxin

Source ID Structure
AlphaFold DB A0A0J1NBD4

References