Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 142737..143263 Replicon plasmid pE28_001
Accession NZ_CP042513
Organism Serratia marcescens strain E28

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag FR888_RS27150 Protein ID WP_000323025.1
Coordinates 142737..143024 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID J5W3H0
Locus tag FR888_RS27155 Protein ID WP_004196370.1
Coordinates 143024..143263 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FR888_RS27115 138408..138725 - 318 WP_015065519.1 hypothetical protein -
FR888_RS27120 138740..139090 - 351 WP_032425551.1 hypothetical protein -
FR888_RS27125 139087..139359 - 273 WP_032425552.1 hypothetical protein -
FR888_RS27135 140099..141211 + 1113 WP_001300563.1 IS4-like element IS421 family transposase -
FR888_RS27140 141406..141564 - 159 WP_014343509.1 type I toxin-antitoxin system Hok family toxin -
FR888_RS27145 141636..142670 + 1035 Protein_160 IS481 family transposase -
FR888_RS27150 142737..143024 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
FR888_RS27155 143024..143263 - 240 WP_004196370.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
FR888_RS27160 143514..143879 + 366 WP_009651956.1 hypothetical protein -
FR888_RS27165 143923..144660 + 738 WP_008460272.1 HupE/UreJ family protein -
FR888_RS27170 144674..145363 + 690 WP_004196322.1 hypothetical protein -
FR888_RS27175 145394..146770 - 1377 Protein_166 chromate efflux transporter -
FR888_RS27180 146727..147704 - 978 WP_004196334.1 chromate resistance protein -
FR888_RS27185 147734..147926 + 193 Protein_168 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..186249 186249


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(3-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T135771 WP_000323025.1 NZ_CP042513:c143024-142737 [Serratia marcescens]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T135771 NZ_CP042513:c143024-142737 [Serratia marcescens]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9018.43 Da        Isoelectric Point: 4.3894

>AT135771 WP_004196370.1 NZ_CP042513:c143263-143024 [Serratia marcescens]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLCNPKPVRVTLDEL

Download         Length: 240 bp

>AT135771 NZ_CP042513:c143263-143024 [Serratia marcescens]
ATGGGTAGCATTAACCTACGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGCGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGACTTTGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4QRH4

References