Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 46819..47555 Replicon plasmid pE28_001
Accession NZ_CP042513
Organism Serratia marcescens strain E28

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag FR888_RS26570 Protein ID WP_003026803.1
Coordinates 47073..47555 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag FR888_RS26565 Protein ID WP_003026799.1
Coordinates 46819..47085 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FR888_RS28680 42405..42605 - 201 WP_023280894.1 hypothetical protein -
FR888_RS26540 43518..43826 - 309 WP_017896554.1 hypothetical protein -
FR888_RS26555 45545..46093 + 549 WP_001567366.1 thioredoxin fold domain-containing protein -
FR888_RS26560 46140..46574 + 435 WP_001567367.1 cell envelope integrity protein TolA -
FR888_RS26565 46819..47085 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
FR888_RS26570 47073..47555 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
FR888_RS26575 47756..49159 + 1404 WP_001567368.1 ISNCY-like element ISKpn21 family transposase -
FR888_RS26580 49188..49820 - 633 WP_001567369.1 hypothetical protein -
FR888_RS26585 50046..51392 + 1347 WP_077253535.1 ISNCY family transposase -
FR888_RS26590 51441..51836 + 396 WP_004143398.1 helix-turn-helix domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..186249 186249
- inside IScluster/Tn - - 43933..53422 9489


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T135770 WP_003026803.1 NZ_CP042513:47073-47555 [Serratia marcescens]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T135770 NZ_CP042513:47073-47555 [Serratia marcescens]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT135770 WP_003026799.1 NZ_CP042513:46819-47085 [Serratia marcescens]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT135770 NZ_CP042513:46819-47085 [Serratia marcescens]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATTCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References