Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 12522..13165 Replicon plasmid pE28_001
Accession NZ_CP042513
Organism Serratia marcescens strain E28

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag FR888_RS26385 Protein ID WP_001044770.1
Coordinates 12749..13165 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag FR888_RS26380 Protein ID WP_001261282.1
Coordinates 12522..12752 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FR888_RS28675 8071..8184 + 114 WP_014343462.1 hypothetical protein -
FR888_RS26335 8313..8570 + 258 WP_009310077.1 hypothetical protein -
FR888_RS26340 8628..9407 - 780 WP_023287113.1 site-specific integrase -
FR888_RS26345 9605..10621 - 1017 WP_009309921.1 hypothetical protein -
FR888_RS26350 10655..10990 - 336 WP_009309920.1 hypothetical protein -
FR888_RS26355 11040..11351 - 312 WP_009309919.1 hypothetical protein -
FR888_RS26360 11409..11714 - 306 WP_004197633.1 type II toxin-antitoxin system toxin CcdB -
FR888_RS26365 11716..11934 - 219 WP_006788213.1 type II toxin-antitoxin system antitoxin CcdA -
FR888_RS26375 12104..12565 - 462 WP_192802327.1 hypothetical protein -
FR888_RS26380 12522..12752 + 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
FR888_RS26385 12749..13165 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
FR888_RS26390 13239..14801 + 1563 WP_004206609.1 AAA family ATPase -
FR888_RS26395 14786..15808 + 1023 WP_151523148.1 DNA helicase UvrD -
FR888_RS26400 16346..17260 + 915 WP_031592169.1 HNH endonuclease -
FR888_RS26405 17446..17796 - 351 WP_004187110.1 DUF305 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..186249 186249


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T135769 WP_001044770.1 NZ_CP042513:12749-13165 [Serratia marcescens]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T135769 NZ_CP042513:12749-13165 [Serratia marcescens]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT135769 WP_001261282.1 NZ_CP042513:12522-12752 [Serratia marcescens]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT135769 NZ_CP042513:12522-12752 [Serratia marcescens]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References