Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 11409..11934 Replicon plasmid pE28_001
Accession NZ_CP042513
Organism Serratia marcescens strain E28

Toxin (Protein)


Gene name ccdB Uniprot ID J5VBT8
Locus tag FR888_RS26360 Protein ID WP_004197633.1
Coordinates 11409..11714 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A1D8K3R4
Locus tag FR888_RS26365 Protein ID WP_006788213.1
Coordinates 11716..11934 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FR888_RS26325 6458..6547 + 90 Protein_7 IS1 family transposase -
FR888_RS28670 6516..6656 + 141 WP_192802328.1 hypothetical protein -
FR888_RS26330 6695..7675 - 981 WP_014386491.1 IS5-like element ISKpn26 family transposase -
FR888_RS28675 8071..8184 + 114 WP_014343462.1 hypothetical protein -
FR888_RS26335 8313..8570 + 258 WP_009310077.1 hypothetical protein -
FR888_RS26340 8628..9407 - 780 WP_023287113.1 site-specific integrase -
FR888_RS26345 9605..10621 - 1017 WP_009309921.1 hypothetical protein -
FR888_RS26350 10655..10990 - 336 WP_009309920.1 hypothetical protein -
FR888_RS26355 11040..11351 - 312 WP_009309919.1 hypothetical protein -
FR888_RS26360 11409..11714 - 306 WP_004197633.1 type II toxin-antitoxin system toxin CcdB Toxin
FR888_RS26365 11716..11934 - 219 WP_006788213.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
FR888_RS26375 12104..12565 - 462 WP_192802327.1 hypothetical protein -
FR888_RS26380 12522..12752 + 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
FR888_RS26385 12749..13165 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin -
FR888_RS26390 13239..14801 + 1563 WP_004206609.1 AAA family ATPase -
FR888_RS26395 14786..15808 + 1023 WP_151523148.1 DNA helicase UvrD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..186249 186249


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11585.27 Da        Isoelectric Point: 6.4661

>T135768 WP_004197633.1 NZ_CP042513:c11714-11409 [Serratia marcescens]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDDSYRLMTTDMASVTASVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T135768 NZ_CP042513:c11714-11409 [Serratia marcescens]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTCTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGACGACAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGGCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8362.31 Da        Isoelectric Point: 4.7405

>AT135768 WP_006788213.1 NZ_CP042513:c11934-11716 [Serratia marcescens]
MKQRITVTIDSDSYQLLKSADVNISGLVNRAMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT135768 NZ_CP042513:c11934-11716 [Serratia marcescens]
ATGAAGCAGCGCATCACCGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCGGACGTCAATATCTCCGGCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCCGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2A5MCG5

Antitoxin

Source ID Structure
AlphaFold DB A0A1D8K3R4

References