Detailed information of TA system TA135746

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 14279..14880
• Replicon: chromosome
• Accession: NZ_CP042512
• Organism: Serratia marcescens strain E28

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: FR888_RS00070
• Protein ID: WP_033636540.1
• Coordinates: 14279..14659 (-)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A0G3SPC0
• Locus tag: FR888_RS00075
• Protein ID: WP_038875999.1
• Coordinates: 14659..14880 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FR888_RS00045	9532..10812	+	1281	WP_055315561.1	DUF3748 domain-containing protein	-
FR888_RS00050	10843..11190	-	348	WP_016929593.1	YceK/YidQ family lipoprotein	-
FR888_RS00055	11500..11913	+	414	WP_004933919.1	heat shock chaperone IbpA	-
FR888_RS00060	12014..12442	+	429	WP_004933922.1	heat shock chaperone IbpB	-
FR888_RS00065	12610..14268	+	1659	WP_047729572.1	putative transporter	-
FR888_RS00070	14279..14659	-	381	WP_033636540.1	type II toxin-antitoxin system VapC family toxin	Toxin
FR888_RS00075	14659..14880	-	222	WP_038875999.1	ribbon-helix-helix domain-containing protein	Antitoxin
FR888_RS00080	14955..16205	-	1251	WP_004933935.1	valine--pyruvate transaminase	-
FR888_RS00085	16341..18404	-	2064	WP_060434093.1	alpha-amylase	-
FR888_RS28390	18600..18752	+	153	WP_168166338.1	hypothetical protein	-
FR888_RS00090	18754..19731	-	978	WP_016929588.1	glyoxylate/hydroxypyruvate reductase GhrB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-102)

Antitoxin

• Ribbon-helix-helix protein, CopG (3-40)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 14185.53 Da
• Isoelectric Point: 7.3170

>T135746 WP_033636540.1 NZ_CP042512:c14659-14279 [Serratia marcescens]
MVAQRALFDTNILIDYLNGIPQAKDVLTEYHINPAISAITWMEVMVGAKKQGPALELKTRQFLGQFLLLPITDEVAERAV
ELRHSQHVKLPDAIIWATAQVGFRTLISRNPKDFGTDNGVLMPYRL

Nucleotide

• Length: 381 bp

>T135746 NZ_CP042512:c14659-14279 [Serratia marcescens]
ATGGTGGCTCAACGGGCGTTATTCGACACCAATATCCTGATCGATTATCTCAACGGCATTCCTCAAGCCAAAGACGTTCT
GACCGAATACCACATCAACCCGGCTATTAGCGCCATCACCTGGATGGAAGTGATGGTGGGTGCCAAAAAACAGGGGCCGG
CGCTGGAGCTGAAAACGCGCCAGTTTTTAGGGCAATTCCTGCTGCTGCCTATTACCGATGAAGTTGCCGAACGCGCCGTA
GAATTGCGCCACTCACAGCATGTGAAGCTGCCGGACGCCATTATCTGGGCCACCGCGCAGGTGGGGTTCCGGACGCTCAT
CTCCCGCAACCCCAAAGATTTTGGCACTGACAACGGCGTGTTAATGCCTTACCGGCTGTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8563.50 Da
• Isoelectric Point: 4.4791

>AT135746 WP_038875999.1 NZ_CP042512:c14880-14659 [Serratia marcescens]
MTRIVIDLPEEDLRQLDNLKNIRHVPRAEIIRQAVSRYLAENHVEDPSNAFGLWSGAMGDGVDYENRQREEWE

Nucleotide

• Length: 222 bp

>AT135746 NZ_CP042512:c14880-14659 [Serratia marcescens]
ATGACTCGCATCGTGATCGATTTACCTGAAGAAGACCTGCGGCAGCTCGACAATCTGAAAAATATCCGCCACGTTCCCCG
GGCCGAAATCATCCGTCAGGCCGTCTCCCGTTATCTGGCGGAGAACCACGTTGAAGATCCCAGCAATGCCTTCGGGCTCT
GGTCCGGGGCGATGGGCGATGGCGTAGACTACGAAAATCGTCAGCGCGAGGAGTGGGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A0G3SPC0