Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 8412..9055 Replicon plasmid pE76_001
Accession NZ_CP042500
Organism Enterobacter sp. E76

Toxin (Protein)


Gene name vagD Uniprot ID A0A5J6WAL0
Locus tag FR762_RS23815 Protein ID WP_061549447.1
Coordinates 8639..9055 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID A0A6P1V8K5
Locus tag FR762_RS23810 Protein ID WP_061549448.1
Coordinates 8412..8642 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FR762_RS23785 4043..4846 - 804 WP_151605696.1 hypothetical protein -
FR762_RS23795 5767..6642 + 876 WP_151605698.1 hypothetical protein -
FR762_RS23800 6706..6891 - 186 WP_061549450.1 hypothetical protein -
FR762_RS23805 7046..7444 + 399 WP_151605699.1 hypothetical protein -
FR762_RS23810 8412..8642 + 231 WP_061549448.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
FR762_RS23815 8639..9055 + 417 WP_061549447.1 type II toxin-antitoxin system VapC family toxin Toxin
FR762_RS23820 9245..9571 + 327 WP_071789783.1 four-helix bundle copper-binding protein -
FR762_RS23825 10355..11263 + 909 WP_151605700.1 HNH endonuclease -
FR762_RS23830 11646..12793 + 1148 WP_142273976.1 IS3 family transposase -
FR762_RS23835 12906..13337 - 432 WP_151605701.1 silver-binding protein SilE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..148871 148871


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-50)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.57 Da        Isoelectric Point: 6.7049

>T135745 WP_061549447.1 NZ_CP042500:8639-9055 [Enterobacter sp. E76]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRNHRIVVSAITYAEMRFGATGPKASPRHIELVDAFCARLDAIQPWDRA
AVDATTEIKVALRLTGTPIGPNDTAIAGHAIAVGAILVTNNTREFERVPGLVLEDWVN

Download         Length: 417 bp

>T135745 NZ_CP042500:8639-9055 [Enterobacter sp. E76]
GTGAACAAAACCTATATGCTGGACACCTGTATCTGCTCGTTCATCATGCGTGAGCAGCCGGAAGCGGTACTGAAGCGCCT
GGAGCAGGCGGTGCTGCGTAACCACCGCATCGTGGTCTCGGCCATTACCTACGCCGAAATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGTCACATTGAGCTGGTTGACGCGTTCTGCGCCCGTCTCGATGCCATCCAGCCCTGGGACCGCGCC
GCGGTGGATGCGACCACAGAGATCAAGGTGGCGCTGCGCCTCACCGGAACGCCGATCGGCCCGAATGACACGGCCATCGC
CGGACATGCGATAGCGGTGGGGGCCATTCTGGTGACAAACAATACCCGGGAATTTGAGCGGGTGCCGGGCCTGGTGCTGG
AAGACTGGGTTAACTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8737.83 Da        Isoelectric Point: 4.3775

>AT135745 WP_061549448.1 NZ_CP042500:8412-8642 [Enterobacter sp. E76]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQLEKAEPDFMAEREDVVSDEGRFDL

Download         Length: 231 bp

>AT135745 NZ_CP042500:8412-8642 [Enterobacter sp. E76]
ATGCGAACAGTTTCTATATTCAAAAACGGTAACAACCGCGCCATTCGCCTCCCCCGTGATCTGGATTTTGAAGGGGTGAG
TGAATTGGAAATTGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGCCCAACCTGGGGATCGTTCGCGCAGCTCG
AAAAGGCTGAACCGGACTTTATGGCGGAGCGTGAGGACGTTGTCAGCGACGAAGGACGATTTGACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5J6WAL0

Antitoxin

Source ID Structure
AlphaFold DB A0A6P1V8K5

References