Detailed information of TA system TA135734

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 2475538..2476159
• Replicon: chromosome
• Accession: NZ_CP042499
• Organism: Enterobacter sp. E76

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: A0A3R9FT17
• Locus tag: FR762_RS11830
• Protein ID: WP_059040068.1
• Coordinates: 2475538..2475756 (-)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: FR762_RS11835
• Protein ID: WP_125294098.1
• Coordinates: 2475785..2476159 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FR762_RS11795	2470655..2471008	+	354	WP_151603831.1	DUF1428 family protein	-
FR762_RS11800	2471016..2471567	-	552	WP_151603833.1	EAL domain-containing protein	-
FR762_RS11810	2472884..2473945	-	1062	WP_151603835.1	CSS-motif domain-containing protein	-
FR762_RS11815	2474189..2474449	+	261	WP_125294095.1	type B 50S ribosomal protein L31	-
FR762_RS11820	2474452..2474592	+	141	WP_125294096.1	type B 50S ribosomal protein L36	-
FR762_RS11825	2474629..2475099	-	471	WP_151603837.1	YlaC family protein	-
FR762_RS11830	2475538..2475756	-	219	WP_059040068.1	hemolysin expression modulator Hha	Toxin
FR762_RS11835	2475785..2476159	-	375	WP_125294098.1	Hha toxicity modulator TomB	Antitoxin
FR762_RS11840	2476630..2479779	-	3150	WP_125294099.1	multidrug efflux RND transporter permease subunit AcrB	-
FR762_RS11845	2479802..2480998	-	1197	WP_125294100.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-116)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8640.05 Da
• Isoelectric Point: 8.9008

>T135734 WP_059040068.1 NZ_CP042499:c2475756-2475538 [Enterobacter sp. E76]
MSEKPLTKIDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Nucleotide

• Length: 219 bp

>T135734 NZ_CP042499:c2475756-2475538 [Enterobacter sp. E76]
ATGTCAGAAAAGCCTTTAACAAAAATTGATTATTTAATGCGTCTACGACGCTGTCAGACGATTGATACGCTTGAGCGGGT
AATTGAGAAAAATAAATACGAATTATCAGATAACGAACTGGCGGTATTTTATTCAGCAGCCGACCACCGTCTCGCCGAAC
TGACGATGAATAAGCTTTACGATAAAATACCGTCTTCTGTGTGGAAATTTATCCGTTAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14341.12 Da
• Isoelectric Point: 4.6864

>AT135734 WP_125294098.1 NZ_CP042499:c2476159-2475785 [Enterobacter sp. E76]
MDEYSPKRHDIAQLKFLCENLYHDCLATLGDSNHGWVNDPTSAINLQLNELIEHIASFALNYKIKYDEDNALIEQIDEYL
DDTFMLFSNYGISAQDLQKWRKSGNRLFKCFVNVSKANPVSLSF

Nucleotide

• Length: 375 bp

>AT135734 NZ_CP042499:c2476159-2475785 [Enterobacter sp. E76]
ATGGACGAATACTCACCAAAACGGCATGATATAGCGCAGCTAAAATTCTTGTGCGAAAATCTGTATCATGACTGCCTGGC
TACGCTTGGTGATAGCAATCACGGCTGGGTCAACGATCCAACCTCGGCCATCAACCTGCAGCTAAATGAATTGATTGAGC
ATATTGCGAGTTTTGCGCTTAATTACAAAATTAAGTATGACGAAGACAATGCGTTGATTGAACAGATCGATGAATACCTC
GATGATACCTTCATGTTGTTTAGCAACTACGGGATAAGCGCCCAGGACTTACAGAAATGGCGCAAATCGGGCAATCGCCT
GTTCAAATGCTTTGTCAACGTTAGTAAGGCCAACCCAGTGAGCCTTTCTTTTTAA

Structures

Toxin

• AlphaFold DB: A0A3R9FT17