Detailed information of TA system TA135727

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 224590..225116
• Replicon: plasmid pC15_001
• Accession: NZ_CP042489
• Organism: Enterobacter hormaechei strain C15

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: FR808_RS24250
• Protein ID: WP_000323025.1
• Coordinates: 224590..224877 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: FR808_RS24255
• Protein ID: WP_000534858.1
• Coordinates: 224877..225116 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FR808_RS25585	220160..220336	-	177	WP_001371930.1	hypothetical protein	-
FR808_RS24220	220847..221791	+	945	WP_000778029.1	DUF5417 domain-containing protein	-
FR808_RS24225	221887..222489	+	603	WP_012695474.1	hypothetical protein	-
FR808_RS24230	222549..222899	+	351	WP_000743059.1	hypothetical protein	-
FR808_RS24235	222946..223149	+	204	WP_001015183.1	hypothetical protein	-
FR808_RS24240	223431..223751	+	321	WP_000332796.1	hypothetical protein	-
FR808_RS24245	224360..224518	-	159	WP_001447866.1	type I toxin-antitoxin system Hok family toxin	-
FR808_RS24250	224590..224877	-	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
FR808_RS24255	224877..225116	-	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
FR808_RS24265	225379..226302	-	924	WP_000167917.1	cation diffusion facilitator family transporter	-
FR808_RS24270	226502..227074	-	573	WP_001515348.1	cytochrome b/b6 domain-containing protein	-
FR808_RS24275	227550..228788	-	1239	WP_000219087.1	IS110 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B / formA / mcr-9 / tet(D) / catA2 / aph(6)-Id / aph(3'')-Ib / dfrA19 / sul1 / qnrA1 / qacE / aadA2 / aac(3)-IId / mph(A) / ARR-3 / catB3 / blaOXA-1 / aac(6')-Ib-cr / blaSHV-12	-	1..340181	340181

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T135727 WP_000323025.1 NZ_CP042489:c224877-224590 [Enterobacter hormaechei]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T135727 NZ_CP042489:c224877-224590 [Enterobacter hormaechei]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT135727 WP_000534858.1 NZ_CP042489:c225116-224877 [Enterobacter hormaechei]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT135727 NZ_CP042489:c225116-224877 [Enterobacter hormaechei]
ATGGGTAGCATTAACCTACGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGCGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGACTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• PDB: 4FXE
• PDB: 2KC8
• PDB: 2K29
• AlphaFold DB: A0A4V1CTS8