Detailed information of TA system TA135708

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/ElaA-DUF1778
• Location: 70610..71413
• Replicon: plasmid pC51_002
• Accession: NZ_CP042483
• Organism: Klebsiella pneumoniae strain C51

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: A0A7W3E3C9
• Locus tag: FR751_RS27220
• Protein ID: WP_022652313.1
• Coordinates: 70883..71413 (+)
• Length: 177 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: W8E6Q5
• Locus tag: FR751_RS27215
• Protein ID: WP_022652312.1
• Coordinates: 70610..70879 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FR751_RS27190	65614..66636	+	1023	WP_032430957.1	IS110 family transposase	-
FR751_RS27195	66928..67235	-	308	Protein_74	IS1 family transposase	-
FR751_RS27205	67712..68439	+	728	Protein_75	site-specific integrase	-
FR751_RS27210	68765..69754	-	990	WP_022652311.1	RepB family plasmid replication initiator protein	-
FR751_RS27215	70610..70879	+	270	WP_022652312.1	DUF1778 domain-containing protein	Antitoxin
FR751_RS27220	70883..71413	+	531	WP_022652313.1	GNAT family N-acetyltransferase	Toxin
FR751_RS27225	71545..72438	+	894	WP_077873527.1	IS5 family transposase	-
FR751_RS27230	72579..72869	-	291	Protein_80	IS3 family transposase	-
FR751_RS27235	72971..73708	-	738	WP_072033343.1	IS21-like element helper ATPase IstB	-
FR751_RS27240	73725..75269	-	1545	WP_043016701.1	IS21 family transposase	-
FR751_RS27245	75509..76117	-	609	Protein_83	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaIMP-4 / catB3 / ARR-3 / qacE / sul1 / mph(A) / aac(3)-IId	-	1..208956	208956
	-	inside	IScluster/Tn	-	-	65614..81624	16010

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (92-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 177 a.a.
• Molecular weight: 20349.26 Da
• Isoelectric Point: 6.7496

>T135708 WP_022652313.1 NZ_CP042483:70883-71413 [Klebsiella pneumoniae]
MEGLRIEIFSEEVEYELSNFDCGEEYLNTFLTDHLKRQHNSKILRGYVLVTRENKPRVMGYYTLSGSCFEKILLPSKTQQ
KRVPYKNVPSVTLGRLAIDKSIHHQGYGETLVTHAMKVVYQASQAVGIHGMFVEALNDNAKKFYLRLGFIQLKEENCNSL
FYPTKSIEELFEVNDE

Nucleotide

• Length: 531 bp

>T135708 NZ_CP042483:70883-71413 [Klebsiella pneumoniae]
ATGGAGGGTTTAAGGATTGAGATTTTTTCAGAGGAAGTTGAATATGAATTAAGCAACTTCGATTGTGGAGAAGAGTATCT
GAATACTTTTCTTACTGACCATCTGAAACGGCAGCATAACAGTAAGATCTTACGTGGTTATGTTCTCGTGACGAGAGAGA
ATAAACCGCGTGTGATGGGGTACTACACGCTATCAGGAAGTTGCTTTGAGAAAATACTTCTACCCAGCAAAACACAACAA
AAGCGTGTACCCTACAAGAATGTGCCGAGCGTGACTTTGGGCCGTCTGGCAATTGATAAAAGCATTCACCACCAGGGATA
TGGAGAAACACTTGTTACCCATGCGATGAAAGTCGTGTATCAGGCCTCGCAGGCTGTGGGTATACACGGAATGTTCGTGG
AAGCGCTGAACGACAACGCCAAAAAATTCTACCTTCGACTGGGTTTCATTCAGTTAAAGGAAGAAAACTGTAACTCGCTC
TTTTACCCGACAAAATCTATTGAAGAATTGTTCGAAGTAAATGATGAATAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10269.70 Da
• Isoelectric Point: 5.7252

>AT135708 WP_022652312.1 NZ_CP042483:70610-70879 [Klebsiella pneumoniae]
MSALKKQRIDLRLNEDDKHMIEEAAAMTNQSISQFMVSTASERAAEVIDQHRRLLLNEESWNLVMDAIINPPAPNDRLKR
AANRLRELE

Nucleotide

• Length: 270 bp

>AT135708 NZ_CP042483:70610-70879 [Klebsiella pneumoniae]
ATGTCAGCACTGAAAAAGCAGCGCATTGACCTGAGATTAAATGAAGACGACAAACACATGATCGAAGAAGCTGCAGCAAT
GACTAACCAGTCAATTTCGCAGTTCATGGTCAGTACCGCCTCAGAACGCGCTGCAGAAGTTATTGACCAGCACCGTCGTC
TGCTCCTCAATGAGGAGTCTTGGAACCTGGTTATGGATGCAATCATTAATCCGCCAGCACCGAATGACCGGCTGAAACGC
GCCGCTAACCGTCTCCGGGAACTGGAGTAA

Structures

Toxin

• AlphaFold DB: A0A7W3E3C9

Antitoxin

• AlphaFold DB: A0A2Z3XDS5