Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/ElaA-DUF1778
Location 70610..71413 Replicon plasmid pC51_002
Accession NZ_CP042483
Organism Klebsiella pneumoniae strain C51

Toxin (Protein)


Gene name KacT Uniprot ID A0A7W3E3C9
Locus tag FR751_RS27220 Protein ID WP_022652313.1
Coordinates 70883..71413 (+) Length 177 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID W8E6Q5
Locus tag FR751_RS27215 Protein ID WP_022652312.1
Coordinates 70610..70879 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FR751_RS27190 65614..66636 + 1023 WP_032430957.1 IS110 family transposase -
FR751_RS27195 66928..67235 - 308 Protein_74 IS1 family transposase -
FR751_RS27205 67712..68439 + 728 Protein_75 site-specific integrase -
FR751_RS27210 68765..69754 - 990 WP_022652311.1 RepB family plasmid replication initiator protein -
FR751_RS27215 70610..70879 + 270 WP_022652312.1 DUF1778 domain-containing protein Antitoxin
FR751_RS27220 70883..71413 + 531 WP_022652313.1 GNAT family N-acetyltransferase Toxin
FR751_RS27225 71545..72438 + 894 WP_077873527.1 IS5 family transposase -
FR751_RS27230 72579..72869 - 291 Protein_80 IS3 family transposase -
FR751_RS27235 72971..73708 - 738 WP_072033343.1 IS21-like element helper ATPase IstB -
FR751_RS27240 73725..75269 - 1545 WP_043016701.1 IS21 family transposase -
FR751_RS27245 75509..76117 - 609 Protein_83 IS3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaIMP-4 / catB3 / ARR-3 / qacE / sul1 / mph(A) / aac(3)-IId - 1..208956 208956
- inside IScluster/Tn - - 65614..81624 16010


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(92-156)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 177 a.a.        Molecular weight: 20349.26 Da        Isoelectric Point: 6.7496

>T135708 WP_022652313.1 NZ_CP042483:70883-71413 [Klebsiella pneumoniae]
MEGLRIEIFSEEVEYELSNFDCGEEYLNTFLTDHLKRQHNSKILRGYVLVTRENKPRVMGYYTLSGSCFEKILLPSKTQQ
KRVPYKNVPSVTLGRLAIDKSIHHQGYGETLVTHAMKVVYQASQAVGIHGMFVEALNDNAKKFYLRLGFIQLKEENCNSL
FYPTKSIEELFEVNDE

Download         Length: 531 bp

>T135708 NZ_CP042483:70883-71413 [Klebsiella pneumoniae]
ATGGAGGGTTTAAGGATTGAGATTTTTTCAGAGGAAGTTGAATATGAATTAAGCAACTTCGATTGTGGAGAAGAGTATCT
GAATACTTTTCTTACTGACCATCTGAAACGGCAGCATAACAGTAAGATCTTACGTGGTTATGTTCTCGTGACGAGAGAGA
ATAAACCGCGTGTGATGGGGTACTACACGCTATCAGGAAGTTGCTTTGAGAAAATACTTCTACCCAGCAAAACACAACAA
AAGCGTGTACCCTACAAGAATGTGCCGAGCGTGACTTTGGGCCGTCTGGCAATTGATAAAAGCATTCACCACCAGGGATA
TGGAGAAACACTTGTTACCCATGCGATGAAAGTCGTGTATCAGGCCTCGCAGGCTGTGGGTATACACGGAATGTTCGTGG
AAGCGCTGAACGACAACGCCAAAAAATTCTACCTTCGACTGGGTTTCATTCAGTTAAAGGAAGAAAACTGTAACTCGCTC
TTTTACCCGACAAAATCTATTGAAGAATTGTTCGAAGTAAATGATGAATAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10269.70 Da        Isoelectric Point: 5.7252

>AT135708 WP_022652312.1 NZ_CP042483:70610-70879 [Klebsiella pneumoniae]
MSALKKQRIDLRLNEDDKHMIEEAAAMTNQSISQFMVSTASERAAEVIDQHRRLLLNEESWNLVMDAIINPPAPNDRLKR
AANRLRELE

Download         Length: 270 bp

>AT135708 NZ_CP042483:70610-70879 [Klebsiella pneumoniae]
ATGTCAGCACTGAAAAAGCAGCGCATTGACCTGAGATTAAATGAAGACGACAAACACATGATCGAAGAAGCTGCAGCAAT
GACTAACCAGTCAATTTCGCAGTTCATGGTCAGTACCGCCTCAGAACGCGCTGCAGAAGTTATTGACCAGCACCGTCGTC
TGCTCCTCAATGAGGAGTCTTGGAACCTGGTTATGGATGCAATCATTAATCCGCCAGCACCGAATGACCGGCTGAAACGC
GCCGCTAACCGTCTCCGGGAACTGGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7W3E3C9

Antitoxin

Source ID Structure
AlphaFold DB A0A2Z3XDS5

References