135707

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 26861..27504 Replicon plasmid pC51_002
Accession NZ_CP042483
Organism Klebsiella pneumoniae strain C51

Toxin (Protein)


Gene name vagD Uniprot ID A0A6C0NE46
Locus tag FR751_RS26960 Protein ID WP_008322233.1
Coordinates 27088..27504 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag FR751_RS26955 Protein ID WP_001261276.1
Coordinates 26861..27091 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FR751_RS26915 23076..23903 + 828 WP_032426659.1 universal stress protein -
FR751_RS26920 23983..24186 + 204 WP_020805555.1 hypothetical protein -
FR751_RS26925 24310..24495 - 186 Protein_24 transposase -
FR751_RS26930 24498..24741 - 244 Protein_25 transposase -
FR751_RS26940 24796..25500 - 705 WP_001067855.1 IS6-like element IS26 family transposase -
FR751_RS26945 25586..26608 + 1023 WP_008322227.1 helicase UvrD -
FR751_RS26955 26861..27091 + 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
FR751_RS26960 27088..27504 + 417 WP_008322233.1 type II toxin-antitoxin system VapC family toxin Toxin
FR751_RS26965 27698..27867 + 170 Protein_30 DUF596 domain-containing protein -
FR751_RS26970 28050..28970 - 921 WP_008322235.1 carbohydrate kinase -
FR751_RS26975 28967..30337 - 1371 WP_008322237.1 hypothetical protein -
FR751_RS26980 30415..31434 - 1020 WP_004113181.1 ribose ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaIMP-4 / catB3 / ARR-3 / qacE / sul1 / mph(A) / aac(3)-IId - 1..208956 208956
- flank IS/Tn - - 24796..25500 704


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15054.58 Da        Isoelectric Point: 9.2957

>T135707 WP_008322233.1 NZ_CP042483:27088-27504 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAASAVLVTNNVREFARVPGLMLEDWVK

Download         Length: 417 bp

>T135707 NZ_CP042483:27088-27504 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTACGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCTGTCCTGCCCTGGGACCGCGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCGGCCAGTGCCGTCCTGGTGACGAACAATGTGAGAGAGTTTGCGCGGGTCCCGGGCCTGATGCTGG
AAGACTGGGTAAAATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT135707 WP_001261276.1 NZ_CP042483:26861-27091 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT135707 NZ_CP055107:210937-211185 [Klebsiella pneumoniae]
ATGGCATTTCAGGTACTAACCACTACTGCAGCAAGCATCACAGAGCTTAAACGTGATCCTATGGGCACTTTCAATGCAGG
AGAAGGTGCCCCGGTAGCCATACTGAATCGCAACGAACCCGCTTTTTACTGTGTACCGCCTGCTCTGTATGCTCAACTGA
TGGAGCTTCTTGAAGATCAGGAGCTGGGTCGTATTGCTGATGAGCGAATTGGTGAGGCGGTAATCGAGGTCAGTCTAGAT
GACCTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6C0NE46

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References