Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 5531..6168 Replicon plasmid pC51_002
Accession NZ_CP042483
Organism Klebsiella pneumoniae strain C51

Toxin (Protein)


Gene name vagD Uniprot ID A0A4P0XKT8
Locus tag FR751_RS26840 Protein ID WP_032426501.1
Coordinates 5758..6168 (+) Length 137 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID A0A4P0XIS7
Locus tag FR751_RS26835 Protein ID WP_020805477.1
Coordinates 5531..5761 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FR751_RS26805 1550..2176 + 627 WP_032426842.1 ParA family plasmid-partitioning AAA ATPase -
FR751_RS28815 2173..2475 + 303 WP_049245955.1 hypothetical protein -
FR751_RS26815 2974..3768 - 795 WP_049245965.1 site-specific integrase -
FR751_RS26820 3765..4586 - 822 WP_020805487.1 hypothetical protein -
FR751_RS26825 4630..4965 - 336 WP_135717592.1 hypothetical protein -
FR751_RS26835 5531..5761 + 231 WP_020805477.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
FR751_RS26840 5758..6168 + 411 WP_032426501.1 PIN domain-containing protein Toxin
FR751_RS26845 6319..6708 - 390 WP_020805483.1 DUF190 domain-containing protein -
FR751_RS26850 6705..8078 - 1374 WP_020805486.1 voltage-gated chloride channel family protein -
FR751_RS26855 8420..8998 - 579 WP_032426503.1 phosphatase PAP2 family protein -
FR751_RS26860 9319..10281 + 963 WP_032426505.1 manganese-dependent inorganic pyrophosphatase -
FR751_RS26865 10285..10713 + 429 WP_020805479.1 universal stress protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaIMP-4 / catB3 / ARR-3 / qacE / sul1 / mph(A) / aac(3)-IId - 1..208956 208956


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-127)

Antitoxin

SpoVT-AbrB domain

(7-44)


Sequences

Toxin        


Download         Length: 137 a.a.        Molecular weight: 14346.31 Da        Isoelectric Point: 5.1082

>T135706 WP_032426501.1 NZ_CP042483:5758-6168 [Klebsiella pneumoniae]
VNRTYMLDTRLCAFIMREQPEAVLGRLEQAVLRGHHIVISAVTWAELSQAARASGPATQALADAFCASLDTVLPWDRAAA
DATTDIKVALRQAGTPIGPNDTAIAGHAIAAGAVLVTRNGGEFERVPGLVLEDWSG

Download         Length: 411 bp

>T135706 NZ_CP042483:5758-6168 [Klebsiella pneumoniae]
GTGAACAGAACGTATATGCTCGACACCCGACTCTGCGCGTTCATCATGCGCGAGCAGCCGGAAGCCGTGCTCGGGCGACT
GGAGCAGGCGGTGCTGCGTGGCCACCATATTGTGATTTCAGCCGTCACCTGGGCAGAACTGTCACAGGCCGCAAGGGCGT
CAGGCCCCGCCACGCAGGCCCTGGCTGATGCGTTCTGTGCCAGTCTCGATACCGTGCTTCCCTGGGACCGGGCGGCAGCG
GACGCCACCACGGATATTAAGGTCGCGCTGCGCCAGGCCGGGACGCCGATCGGCCCCAACGACACGGCGATAGCCGGGCA
CGCCATTGCCGCCGGCGCTGTGCTGGTGACCAGGAACGGGGGAGAGTTTGAGCGGGTCCCGGGTCTGGTGCTGGAAGACT
GGTCCGGTTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8591.70 Da        Isoelectric Point: 5.1231

>AT135706 WP_020805477.1 NZ_CP042483:5531-5761 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELESVREGDRIILRPVRPTWGSFALMGKAGPEFMAEREGVISDNGRDEQ

Download         Length: 231 bp

>AT135706 NZ_CP042483:5531-5761 [Klebsiella pneumoniae]
ATGAGAACCGTATCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGCGATCTGGATTTTGAAGGGGTGAG
CGAGCTGGAGAGCGTCCGGGAGGGGGACCGCATCATCCTGCGTCCCGTCCGGCCGACCTGGGGCTCATTCGCCCTGATGG
GTAAAGCCGGGCCTGAGTTTATGGCGGAGCGTGAAGGCGTAATCAGCGATAACGGGCGGGATGAACAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P0XKT8

Antitoxin

Source ID Structure
AlphaFold DB A0A4P0XIS7

References