Detailed information of TA system TA135703

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 131843..132753
• Replicon: plasmid pC51_001
• Accession: NZ_CP042482
• Organism: Klebsiella pneumoniae strain C51

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: FR751_RS26315
• Protein ID: WP_151675976.1
• Coordinates: 131843..132313 (-)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A7H4PRG4
• Locus tag: FR751_RS26320
• Protein ID: WP_032413443.1
• Coordinates: 132310..132753 (-)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FR751_RS26300	128030..129775	+	1746	WP_151675973.1	ATP-grasp domain-containing protein	-
FR751_RS26310	130494..131724	+	1231	Protein_125	IS3 family transposase	-
FR751_RS26315	131843..132313	-	471	WP_151675976.1	RES family NAD+ phosphorylase	Toxin
FR751_RS26320	132310..132753	-	444	WP_032413443.1	DUF2384 domain-containing protein	Antitoxin
FR751_RS26325	132816..133784	-	969	WP_000654811.1	IS5 family transposase	-
FR751_RS26330	133764..134729	+	966	WP_151675979.1	IS5 family transposase	-
FR751_RS28800	134777..135079	-	303	WP_164876979.1	hypothetical protein	-
FR751_RS26335	135149..135568	-	420	WP_011251286.1	helix-turn-helix domain-containing protein	-
FR751_RS26340	135565..135876	-	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	-
FR751_RS26345	136081..136539	-	459	Protein_133	DDE-type integrase/transposase/recombinase	-
FR751_RS26350	136632..136970	+	339	WP_023288266.1	IS66 family insertion sequence hypothetical protein	-
FR751_RS26355	136967..137314	+	348	WP_032430752.1	IS66 family insertion sequence element accessory protein TnpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	formA	cheD	1..216942	216942
	-	inside	IScluster/Tn	formA	cheD	130494..160726	30232

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre (16-147)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17563.79 Da
• Isoelectric Point: 4.5152

>T135703 WP_151675976.1 NZ_CP042482:c132313-131843 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPDNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHSEFYGIVQKAQQIPFRFDSRLKPDRK

Nucleotide

• Length: 471 bp

>T135703 NZ_CP042482:c132313-131843 [Klebsiella pneumoniae]
GTGATCCTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
CTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACGCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATCCAAAGCGCAAACATGGAT
GAATTACCGGACAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAATTATCTGTTAAATCCTGAGCACTCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16508.82 Da
• Isoelectric Point: 10.3013

>AT135703 WP_032413443.1 NZ_CP042482:c132753-132310 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT135703 NZ_CP042482:c132753-132310 [Klebsiella pneumoniae]
ATGAAAACATTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
CGTAGCGCTTCTGGGCCAAATAAATGAAGGTCTGGACGGGAAGGTGGCGAACCGGATAACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCAACCACATTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGTATTATCCGCGTCATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAGGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAAACGGGAGCCTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA

Structures

Antitoxin

• AlphaFold DB: A0A7H4PRG4