Detailed information of TA system TA135684

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/ElaA-DUF1778
• Location: 103221..104024
• Replicon: plasmid pC50_002
• Accession: NZ_CP042480
• Organism: Citrobacter freundii strain C50

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: A0A7W3E3C9
• Locus tag: FR753_RS26895
• Protein ID: WP_022652313.1
• Coordinates: 103494..104024 (+)
• Length: 177 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: W8E6Q5
• Locus tag: FR753_RS26890
• Protein ID: WP_022652312.1
• Coordinates: 103221..103490 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FR753_RS26870	99526..99833	-	308	Protein_102	IS1 family transposase	-
FR753_RS26880	100310..101050	+	741	WP_022652310.1	site-specific integrase	-
FR753_RS26885	101376..102365	-	990	WP_022652311.1	RepB family plasmid replication initiator protein	-
FR753_RS26890	103221..103490	+	270	WP_022652312.1	DUF1778 domain-containing protein	Antitoxin
FR753_RS26895	103494..104024	+	531	WP_022652313.1	GNAT family N-acetyltransferase	Toxin
FR753_RS26900	104156..105049	+	894	WP_077873527.1	IS5 family transposase	-
FR753_RS26905	105190..105480	-	291	Protein_108	IS3 family transposase	-
FR753_RS26910	105582..106319	-	738	WP_072033343.1	IS21-like element helper ATPase IstB	-
FR753_RS26915	106336..107880	-	1545	WP_043016701.1	IS21 family transposase	-
FR753_RS26920	108120..108728	-	609	Protein_111	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaIMP-4 / catB3 / blaOXA-10 / ant(3'')-Ia / blaVEB-3 / aac(6')-Ib-cr / blaOXA-1 / ARR-3 / qacE / sul1 / mph(A) / aac(3)-IId	-	1..176400	176400
	-	inside	IScluster/Tn	-	-	98212..114235	16023

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (92-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 177 a.a.
• Molecular weight: 20349.26 Da
• Isoelectric Point: 6.7496

>T135684 WP_022652313.1 NZ_CP042480:103494-104024 [Citrobacter freundii]
MEGLRIEIFSEEVEYELSNFDCGEEYLNTFLTDHLKRQHNSKILRGYVLVTRENKPRVMGYYTLSGSCFEKILLPSKTQQ
KRVPYKNVPSVTLGRLAIDKSIHHQGYGETLVTHAMKVVYQASQAVGIHGMFVEALNDNAKKFYLRLGFIQLKEENCNSL
FYPTKSIEELFEVNDE

Nucleotide

• Length: 531 bp

>T135684 NZ_CP042480:103494-104024 [Citrobacter freundii]
ATGGAGGGTTTAAGGATTGAGATTTTTTCAGAGGAAGTTGAATATGAATTAAGCAACTTCGATTGTGGAGAAGAGTATCT
GAATACTTTTCTTACTGACCATCTGAAACGGCAGCATAACAGTAAGATCTTACGTGGTTATGTTCTCGTGACGAGAGAGA
ATAAACCGCGTGTGATGGGGTACTACACGCTATCAGGAAGTTGCTTTGAGAAAATACTTCTACCCAGCAAAACACAACAA
AAGCGTGTACCCTACAAGAATGTGCCGAGCGTGACTTTGGGCCGTCTGGCAATTGATAAAAGCATTCACCACCAGGGATA
TGGAGAAACACTTGTTACCCATGCGATGAAAGTCGTGTATCAGGCCTCGCAGGCTGTGGGTATACACGGAATGTTCGTGG
AAGCGCTGAACGACAACGCCAAAAAATTCTACCTTCGACTGGGTTTCATTCAGTTAAAGGAAGAAAACTGTAACTCGCTC
TTTTACCCGACAAAATCTATTGAAGAATTGTTCGAAGTAAATGATGAATAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10269.70 Da
• Isoelectric Point: 5.7252

>AT135684 WP_022652312.1 NZ_CP042480:103221-103490 [Citrobacter freundii]
MSALKKQRIDLRLNEDDKHMIEEAAAMTNQSISQFMVSTASERAAEVIDQHRRLLLNEESWNLVMDAIINPPAPNDRLKR
AANRLRELE

Nucleotide

• Length: 270 bp

>AT135684 NZ_CP042480:103221-103490 [Citrobacter freundii]
ATGTCAGCACTGAAAAAGCAGCGCATTGACCTGAGATTAAATGAAGACGACAAACACATGATCGAAGAAGCTGCAGCAAT
GACTAACCAGTCAATTTCGCAGTTCATGGTCAGTACCGCCTCAGAACGCGCTGCAGAAGTTATTGACCAGCACCGTCGTC
TGCTCCTCAATGAGGAGTCTTGGAACCTGGTTATGGATGCAATCATTAATCCGCCAGCACCGAATGACCGGCTGAAACGC
GCCGCTAACCGTCTCCGGGAACTGGAGTAA

Structures

Toxin

• AlphaFold DB: A0A7W3E3C9

Antitoxin

• AlphaFold DB: A0A2Z3XDS5