135681

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 4903236..4903852 Replicon chromosome
Accession NZ_CP042478
Organism Citrobacter freundii strain C50

Toxin (Protein)


Gene name vapC Uniprot ID A0A0J1MQ96
Locus tag FR753_RS23995 Protein ID WP_003028682.1
Coordinates 4903236..4903610 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A6B5NTK4
Locus tag FR753_RS24000 Protein ID WP_043018956.1
Coordinates 4903610..4903852 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FR753_RS23980 4900739..4901641 + 903 WP_106107909.1 formate dehydrogenase O subunit beta -
FR753_RS23985 4901638..4902273 + 636 WP_003028686.1 formate dehydrogenase cytochrome b556 subunit -
FR753_RS23990 4902270..4903199 + 930 WP_003028685.1 formate dehydrogenase accessory protein FdhE -
FR753_RS23995 4903236..4903610 - 375 WP_003028682.1 type II toxin-antitoxin system VapC family toxin Toxin
FR753_RS24000 4903610..4903852 - 243 WP_043018956.1 ribbon-helix-helix domain-containing protein Antitoxin
FR753_RS24005 4904058..4904966 + 909 WP_119174684.1 alpha/beta hydrolase -
FR753_RS24015 4905117..4906058 - 942 WP_003825286.1 fatty acid biosynthesis protein FabY -
FR753_RS24020 4906103..4906540 - 438 WP_003028671.1 D-tyrosyl-tRNA(Tyr) deacylase -
FR753_RS24025 4906537..4907409 - 873 WP_003840437.1 virulence factor BrkB family protein -
FR753_RS24030 4907403..4908002 - 600 WP_016151258.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Ribbon-helix-helix protein, CopG

(13-47)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13674.89 Da        Isoelectric Point: 9.5622

>T135681 WP_003028682.1 NZ_CP042478:c4903610-4903236 [Citrobacter freundii]
MVKGSALFDTNILIDLFSGRNEAKLAIETWPPQNAISLITWMEVMVGAKKYHQEARTRVALGAFNVIGVSQDIAERSVSI
RQEYGMKLPDAIILATAQIHRLTLVTRNTKDFAGLSGVVTPYTL

Download         Length: 375 bp

>T135681 NZ_CP042478:c4903610-4903236 [Citrobacter freundii]
ATGGTCAAAGGATCCGCGCTTTTTGATACCAACATCCTCATCGATCTGTTTAGTGGGAGAAATGAAGCAAAACTAGCGAT
AGAAACATGGCCGCCGCAGAATGCAATCAGCCTCATTACATGGATGGAGGTGATGGTGGGTGCAAAAAAATACCATCAGG
AAGCCCGTACTCGTGTTGCGCTAGGTGCTTTTAACGTGATCGGCGTTTCACAGGATATTGCTGAACGAAGCGTGAGTATC
AGACAGGAATATGGGATGAAATTGCCGGATGCCATTATTTTGGCAACAGCACAGATTCATCGTCTTACGCTGGTTACGCG
AAATACTAAGGATTTTGCAGGCCTATCCGGTGTGGTCACACCGTATACATTATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9451.75 Da        Isoelectric Point: 4.3437

>AT135681 WP_043018956.1 NZ_CP042478:c4903852-4903610 [Citrobacter freundii]
MMAGMDMGRILLDLSDEVIKRLDDLKVQRNLPRAELLREAVEQYLERQDQAETTISRALGLWQGCEEDGVEYQRKLREEW

Download         Length: 243 bp

>AT135681 NZ_CP042478:c4903852-4903610 [Citrobacter freundii]
ATGATGGCTGGGATGGATATGGGCAGAATTTTACTCGATTTATCAGATGAAGTGATTAAACGTCTTGATGATCTCAAGGT
GCAGCGCAACCTGCCGCGAGCAGAACTTTTACGGGAGGCCGTTGAACAGTATCTTGAGAGGCAGGATCAGGCTGAAACCA
CAATTTCACGTGCTCTTGGGCTGTGGCAAGGCTGTGAGGAAGATGGTGTTGAATACCAGCGTAAACTCCGTGAGGAATGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J1MQ96

Antitoxin

Source ID Structure
AlphaFold DB A0A6B5NTK4

References