Detailed information of TA system TA135680

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4597590..4598393
• Replicon: chromosome
• Accession: NZ_CP042478
• Organism: Citrobacter freundii strain C50

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A6N6JYU3
• Locus tag: FR753_RS22580
• Protein ID: WP_024156451.1
• Coordinates: 4598013..4598393 (+)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A5A9BSL8
• Locus tag: FR753_RS22575
• Protein ID: WP_038807908.1
• Coordinates: 4597590..4597955 (+)
• Length: 122 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FR753_RS22530	4592656..4593543	+	888	WP_048242072.1	50S ribosome-binding GTPase	-
FR753_RS22535	4593749..4594150	+	402	WP_048242038.1	hypothetical protein	-
FR753_RS22540	4594247..4594714	+	468	WP_048242036.1	hypothetical protein	-
FR753_RS22545	4594905..4595165	+	261	WP_048242035.1	DUF905 family protein	-
FR753_RS22550	4595281..4596099	+	819	WP_048242033.1	DUF945 domain-containing protein	-
FR753_RS22560	4596368..4596841	+	474	WP_024156447.1	antirestriction protein	-
FR753_RS22565	4596854..4597333	+	480	WP_024156448.1	DNA repair protein RadC	-
FR753_RS22570	4597347..4597568	+	222	WP_024156449.1	DUF987 domain-containing protein	-
FR753_RS22575	4597590..4597955	+	366	WP_038807908.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
FR753_RS22580	4598013..4598393	+	381	WP_024156451.1	TA system toxin CbtA family protein	Toxin
FR753_RS22585	4598390..4598881	+	492	WP_024156452.1	hypothetical protein	-
FR753_RS22595	4599190..4600037	+	848	Protein_4338	DUF4942 domain-containing protein	-
FR753_RS22605	4600827..4602488	+	1662	WP_048234127.1	fatty acid--CoA ligase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-110)

Antitoxin

• Antitoxin CbeA (15-114)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 14263.44 Da
• Isoelectric Point: 10.0919

>T135680 WP_024156451.1 NZ_CP042478:4598013-4598393 [Citrobacter freundii]
MQTLSAIPKRKAPSRPTPVEIWQQLLTYLLKRHYGLSLSDTQFSDEEIITQYIDAGISLSDALNFLVEKSELVRIDRPGF
SIKHQSPFIGVIDILRARRATGLMQRNGYKRITLLIAGNAAQEQHS

Nucleotide

• Length: 381 bp

>T135680 NZ_CP042478:4598013-4598393 [Citrobacter freundii]
ATGCAAACCTTATCCGCCATCCCGAAGCGGAAGGCACCGTCACGCCCGACGCCCGTTGAAATCTGGCAGCAACTTCTTAC
GTATCTGTTAAAAAGACATTACGGCCTTTCGCTTAGCGACACGCAATTTTCCGATGAGGAGATTATTACGCAATATATTG
ACGCCGGTATCTCACTGTCAGATGCACTCAATTTTCTCGTTGAAAAATCTGAACTGGTGCGGATTGATCGTCCCGGTTTC
TCCATTAAGCATCAGTCCCCTTTTATCGGGGTGATTGATATTCTCCGGGCCAGACGGGCAACGGGTTTGATGCAACGTAA
CGGGTATAAAAGGATCACTCTTCTCATCGCTGGAAATGCAGCTCAGGAGCAACATTCATGA

Antitoxin

Protein

• Length: 122 a.a.
• Molecular weight: 13344.20 Da
• Isoelectric Point: 5.8705

>AT135680 WP_038807908.1 NZ_CP042478:4597590-4597955 [Citrobacter freundii]
MSNPIPPVNHDVSEPWWGLKPGITPCFGARLVQEGNRLHYLADRASIAGVFSDADLRHLDQAFPVLLKQLELMLVSGELN
PCHQHCVTLYAKGLTCEADSLGSHGYIYTAIYPTPDDSITR

Nucleotide

• Length: 366 bp

>AT135680 NZ_CP042478:4597590-4597955 [Citrobacter freundii]
TTGTCGAATCCAATACCTCCAGTAAACCATGATGTCTCTGAGCCCTGGTGGGGGCTGAAACCGGGCATCACTCCGTGTTT
TGGCGCACGACTGGTACAGGAAGGCAACCGCCTGCATTATCTGGCTGATCGGGCCAGTATCGCCGGTGTGTTCAGCGATG
CGGATTTACGCCATCTCGATCAAGCATTTCCGGTACTGCTCAAACAGCTGGAATTAATGCTGGTTTCCGGTGAGCTCAAT
CCCTGCCATCAGCACTGTGTCACGTTATATGCAAAAGGGCTGACCTGTGAAGCAGATTCCCTCGGATCACATGGCTACAT
TTATACAGCAATTTATCCGACACCAGACGACAGCATAACCCGGTAA

Structures

Toxin

• AlphaFold DB: A0A6N6JYU3

Antitoxin

• AlphaFold DB: A0A5A9BSL8