135678

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3764519..3765139 Replicon chromosome
Accession NZ_CP042478
Organism Citrobacter freundii strain C50

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag FR753_RS18600 Protein ID WP_002892050.1
Coordinates 3764921..3765139 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID R8WZW8
Locus tag FR753_RS18595 Protein ID WP_003021733.1
Coordinates 3764519..3764893 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FR753_RS18585 3759665..3760858 + 1194 WP_003835922.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
FR753_RS18590 3760881..3764030 + 3150 WP_003021736.1 multidrug efflux RND transporter permease subunit -
FR753_RS18595 3764519..3764893 + 375 WP_003021733.1 Hha toxicity modulator TomB Antitoxin
FR753_RS18600 3764921..3765139 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
FR753_RS18605 3765446..3765997 + 552 WP_003835924.1 maltose O-acetyltransferase -
FR753_RS18610 3766114..3766584 + 471 WP_087879033.1 YlaC family protein -
FR753_RS18615 3766663..3766803 - 141 WP_005125190.1 type B 50S ribosomal protein L36 -
FR753_RS18620 3766805..3767065 - 261 WP_003021719.1 type B 50S ribosomal protein L31 -
FR753_RS18625 3767254..3768807 + 1554 WP_003835927.1 EAL domain-containing protein -
FR753_RS18630 3768859..3769212 - 354 WP_003831033.1 DUF1428 family protein -
FR753_RS18635 3769277..3769906 - 630 WP_003835929.1 membrane protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T135678 WP_002892050.1 NZ_CP042478:3764921-3765139 [Citrobacter freundii]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T135678 NZ_CP042478:3764921-3765139 [Citrobacter freundii]
ATGTCCGATAAACCATTAACTAAAACAGATTATTTGATGCGCCTGCGACGTTGCCAGACAATTGATACGCTAGAGCGCGT
TATTGAAAAAAATAAATATGAATTGTCCGACAACGAACTGGCTGTATTTTACTCAGCAGCAGATCATCGTCTTGCTGAAT
TGACCATGAATAAGCTTTACGACAAGATTCCAACTTCTGTTTGGAAATTCATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14427.23 Da        Isoelectric Point: 5.5653

>AT135678 WP_003021733.1 NZ_CP042478:3764519-3764893 [Citrobacter freundii]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEQSNHGWVNDPTSATSLQLNELIEHIATFALNYKIKYNEDNKLIAQIDEYL
DDTFMLFSSYGINTQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Download         Length: 375 bp

>AT135678 NZ_CP042478:3764519-3764893 [Citrobacter freundii]
ATGGATGAATACTCACCCAAAAGACATGATATCGCGCAGCTTAAATTTCTCTGCGAAACTCTGTATCATGACTGTCTTGC
AAACCTTGAACAAAGTAACCATGGCTGGGTTAATGACCCTACCTCTGCAACCAGCTTGCAGCTCAATGAACTGATAGAGC
ATATTGCAACCTTCGCACTTAATTATAAAATTAAGTACAATGAGGACAATAAGCTGATCGCGCAGATAGATGAATACCTG
GATGACACTTTTATGTTGTTCAGCAGTTATGGTATTAATACGCAGGATCTGCAGAAATGGCGGAAGTCAGGTAATCGACT
GTTTCGCTGTTTTGTCAATGCCACCAGGGCTAATCCTGTTAGTTTGTCTTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB R8WZW8

References