Detailed information of TA system TA135676

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2513923..2514562
• Replicon: chromosome
• Accession: NZ_CP042478
• Organism: Citrobacter freundii strain C50

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: FR753_RS12460
• Protein ID: WP_071684398.1
• Coordinates: 2513923..2514099 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: FR753_RS12465
• Protein ID: WP_123924900.1
• Coordinates: 2514146..2514562 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FR753_RS12440	2510701..2510931	-	231	WP_003020233.1	DUF2554 family protein	-
FR753_RS12445	2511121..2511246	-	126	WP_003020230.1	DUF2474 domain-containing protein	-
FR753_RS12450	2511246..2512256	-	1011	WP_003020227.1	cytochrome d ubiquinol oxidase subunit II	-
FR753_RS12455	2512256..2513659	-	1404	WP_003843730.1	cytochrome ubiquinol oxidase subunit I	-
FR753_RS12460	2513923..2514099	+	177	WP_071684398.1	type II toxin-antitoxin system HicA family toxin	Toxin
FR753_RS12465	2514146..2514562	+	417	WP_123924900.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
FR753_RS12470	2514655..2516064	+	1410	WP_003836140.1	PLP-dependent aminotransferase family protein	-
FR753_RS12475	2516392..2517537	+	1146	WP_151532659.1	ABC transporter substrate-binding protein	-
FR753_RS12480	2517554..2518570	+	1017	WP_119174242.1	ABC transporter ATP-binding protein	-
FR753_RS12485	2518571..2519515	+	945	WP_003843727.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (6-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-65)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6768.86 Da
• Isoelectric Point: 11.6208

>T135676 WP_071684398.1 NZ_CP042478:2513923-2514099 [Citrobacter freundii]
VKQSKFRRWLESQGVAVTNGSNHLKLRYQGRRSVMPRHPGDEIKEALRKAIIKQLDLQ

Nucleotide

• Length: 177 bp

>T135676 NZ_CP042478:2513923-2514099 [Citrobacter freundii]
GTGAAGCAAAGTAAGTTTAGACGCTGGCTTGAATCTCAGGGAGTTGCTGTTACAAACGGCTCAAATCATCTGAAACTGAG
ATATCAGGGAAGGCGTAGCGTTATGCCCAGACATCCAGGTGATGAAATAAAAGAAGCCTTGCGAAAAGCGATTATTAAGC
AGCTTGACTTACAGTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15126.36 Da
• Isoelectric Point: 4.5716

>AT135676 WP_123924900.1 NZ_CP042478:2514146-2514562 [Citrobacter freundii]
MRYPVTLTPAVEGGFVVSFPDIPEALTQGNTRHDALQAAQAALITAFEFYFDDNEAIPLPSAVSAEDDYVEIPLSVASKV
LLLNAFLESKITQQELANRIGRPKQEITRLFDLKHTTKIDAVQIAARALGKELALTML

Nucleotide

• Length: 417 bp

>AT135676 NZ_CP042478:2514146-2514562 [Citrobacter freundii]
ATGCGTTATCCTGTTACTCTAACACCGGCAGTGGAAGGTGGGTTTGTGGTTTCTTTCCCGGATATTCCGGAAGCATTAAC
TCAGGGAAATACTCGCCATGACGCTTTGCAGGCCGCGCAGGCGGCATTGATTACCGCTTTTGAATTCTATTTCGACGACA
ACGAGGCGATTCCACTCCCTTCGGCGGTTAGCGCTGAAGATGACTACGTAGAAATACCGCTGAGCGTTGCGTCTAAGGTT
CTTCTGCTGAATGCCTTCCTTGAATCAAAAATCACCCAGCAGGAGCTGGCGAACAGGATCGGCAGACCTAAGCAGGAGAT
CACCCGTTTATTTGATTTAAAGCACACGACAAAAATTGATGCCGTGCAGATTGCTGCGCGCGCCTTAGGCAAAGAGCTGG
CGTTAACGATGTTGTAA